9I0F

Revisited AvNifEN crystal structure

9I0F の概要

• エントリーDOI: 10.2210/pdb9i0f/pdb
• 分子名称: Nitrogenase iron-molybdenum cofactor biosynthesis protein NifE, TETRAETHYLENE GLYCOL, Nitrogenase iron-molybdenum cofactor biosynthesis protein NifN, ... (11 entities in total)
• 機能のキーワード: nitrogenase cofactor maturation, protein binding, metalloenzyme, iron-sulfur cluster, metal binding protein
• 由来する生物種: Azotobacter vinelandii DJ; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 426204.52

構造登録者

Paya Tormo, L.,Nguyen, T.Q.,Fyfe, C.,Basbous, H.,Dobrzynska, K.,Echavarri-Erasun, C.,Martin, L.,Caserta, G.,Legrand, P.,Thorn, A.,Amara, P.,Schoehn, G.,Cherrier, M.V.,Rubio, L.M.,Nicolet, Y. (登録日: 2025-01-15, 公開日: 2025-12-03)

主引用文献

Paya Tormo, L.,Nguyen, T.Q.,Fyfe, C.,Basbous, H.,Dobrzynska, K.,Echavarri-Erasun, C.,Martin, L.,Caserta, G.,Legrand, P.,Thorn, A.,Amara, P.,Schoehn, G.,Cherrier, M.V.,Rubio, L.M.,Nicolet, Y.
Dynamics driving the precursor in NifEN scaffold during nitrogenase FeMo-cofactor assembly.
Nat.Chem.Biol., 2025

PubMed Abstract: Nitrogenase catalyzes atmospheric nitrogen fixation, a critical biological process that depends on an intricate organometallic cofactor assembled by a dedicated multiprotein system. Here we uncover the structural basis for the function of NifEN, the scaffold protein that mediates the final stages of cofactor biosynthesis before its incorporation into nitrogenase. High-resolution structural analyses reveal that the cofactor precursor initially binds at a surface docking site before being transferred into a specialized cavity for further maturation. This process involves dynamic structural rearrangements, including coordinated domain motions and partial unfolding, enabling the scaffold to alternate between open and closed states. Additionally, a rear channel extends to the precursor-binding cavity, likely facilitating the entry of the modifying components molybdenum and homocitrate. These findings illuminate the dynamic mechanisms underlying FeMo-cofactor assembly and underscore the functional divergence between NifEN, the biosynthetic scaffold, and NifDK, the catalytic component of nitrogenase.

PubMed: 41238839
DOI: 10.1038/s41589-025-02070-4

実験手法

X-RAY DIFFRACTION (2.4 Å)