9HYE

Structure of the Mus musclus Langerin carbohydrate recognition domain with depleted Calcium

9HYE の概要

• エントリーDOI: 10.2210/pdb9hye/pdb
• 分子名称: C-type lectin domain family 4 member K, CHLORIDE ION (3 entities in total)
• 機能のキーワード: c-type lectin, glycoprotein, carbohydrate binding protein, calcium binding, crd domain, lectin, immune system
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 35996.16

構造登録者

Ruwolt, M.,Neu, U.,Loll, B. (登録日: 2025-01-09, 公開日: 2025-11-19, 最終更新日: 2025-12-24)

主引用文献

Ning, Y.,Efrem, N.L.,Amoussa, M.,Turhan, E.,Zheng, D.,Lefebre, J.,Ruwolt, M.,Neu, U.,Besch, M.,Loll, B.,Kurzbach, D.,Kohnke, J.,Nazare, M.,Rademacher, C.
Calcium Competitive Inhibition of Langerin by Thiazolopyrimidinones.
J.Med.Chem., 68:24924-24934, 2025

PubMed Abstract: C-Type lectins are a large family of carbohydrate-binding proteins. Langerin is a member of this family and is expressed by Langerhans cells, involved in pathogen recognition and innate immune activation, making it a target for small-molecule modulation in immunology and infectious diseases. We previously identified thiazolopyrimidinones as a series of allosteric inhibitors, but the underlying mechanism remained unclear. In this study, Ca NMR demonstrated that these fragments induce Ca release from the receptor. Our ITC data suggested a competitive relationship between inhibitors and Ca, which was further validated by F NMR spectroscopy showing inhibition of carbohydrate binding. Surprisingly, the fragment binding site was found to be located beneath the long loop, which supports the dynamic nature of the long loop being highly Ca dependent. Our findings provide insight into the novel Ca-competitive inhibitory mechanism of murine langerin and are the first report on such an inhibitory mechanism for a C-type lectin.

PubMed: 41261040
DOI: 10.1021/acs.jmedchem.5c01756

実験手法

X-RAY DIFFRACTION (1.64 Å)