9HQ7

Partial (52mer) encapsulin shell assembly from Mycobacterium tuberculosis

これはPDB形式変換不可エントリーです。

9HQ7 の概要

• エントリーDOI: 10.2210/pdb9hq7/pdb
• 関連するPDBエントリー: 7P1T
• EMDBエントリー: 13164 52340
• 分子名称: Type 1 encapsulin shell protein (1 entity in total)
• 機能のキーワード: encapsulin, virus like particle
• 由来する生物種: Mycobacterium tuberculosis H37Rv
• タンパク質・核酸の鎖数: 52
• 化学式量合計: 1500893.99

構造登録者

Lewis, C.J.,Berger, C.,Ravelli, R.B.G. (登録日: 2024-12-16, 公開日: 2025-02-05, 最終更新日: 2025-02-26)

主引用文献

Berger, C.,Lewis, C.,Gao, Y.,Knoops, K.,Lopez-Iglesias, C.,Peters, P.J.,Ravelli, R.B.G.
In situ and in vitro cryo-EM reveal structures of mycobacterial encapsulin assembly intermediates.
Commun Biol, 8:245-245, 2025

PubMed Abstract: Prokaryotes rely on proteinaceous compartments such as encapsulin to isolate harmful reactions. Encapsulin are widely expressed by bacteria, including the Mycobacteriaceae, which include the human pathogens Mycobacterium tuberculosis and Mycobacterium leprae. Structures of fully assembled encapsulin shells have been determined for several species, but encapsulin assembly and cargo encapsulation are still poorly characterised, because of the absence of encapsulin structures in intermediate assembly states. We combine in situ and in vitro structural electron microscopy to show that encapsulins are dynamic assemblies with intermediate states of cargo encapsulation and shell assembly. Using cryo-focused ion beam (FIB) lamella preparation and cryo-electron tomography (CET), we directly visualise encapsulins in Mycobacterium marinum, and observed ribbon-like attachments to the shell, encapsulin shells with and without cargoes, and encapsulin shells in partially assembled states. In vitro cryo-electron microscopy (EM) single-particle analysis of the Mycobacterium tuberculosis encapsulin was used to obtain three structures of the encapsulin shell in intermediate states, as well as a 2.3 Å structure of the fully assembled shell. Based on the analysis of the intermediate encapsulin shell structures, we propose a model of encapsulin self-assembly via the pairwise addition of monomers.

PubMed: 39955411
DOI: 10.1038/s42003-025-07660-5

実験手法

ELECTRON MICROSCOPY (4.51 Å)