9HC4

Cryo-EM structure of P. urativorans 70S ribosome with 2 copies of bS20.

これはPDB形式変換不可エントリーです。

9HC4 の概要

• エントリーDOI: 10.2210/pdb9hc4/pdb
• EMDBエントリー: 52036
• 分子名称: Small ribosomal subunit protein uS9, Large ribosomal subunit protein bL17, Large ribosomal subunit protein bL25, ... (52 entities in total)
• 機能のキーワード: ribosome, ribosome heterogeneity, functionally specialized ribosomes, polysome
• 由来する生物種: Psychrobacter urativorans; 詳細
• タンパク質・核酸の鎖数: 53
• 化学式量合計: 2180733.71

構造登録者

Helena-Bueno, K.,Hill, C.H.,Melnikov, S.V. (登録日: 2024-11-08, 公開日: 2025-03-26, 最終更新日: 2025-04-02)

主引用文献

Helena-Bueno, K.,Kopetschke, S.,Filbeck, S.,Chan, L.I.,Birsan, S.,Basle, A.,Hudson, M.,Pfeffer, S.,Hill, C.H.,Melnikov, S.V.
Structurally heterogeneous ribosomes cooperate in protein synthesis in bacterial cells.
Nat Commun, 16:2751-2751, 2025

PubMed Abstract: Ribosome heterogeneity is a paradigm in biology, pertaining to the existence of structurally distinct populations of ribosomes within a single organism or cell. This concept suggests that structurally distinct pools of ribosomes have different functional properties and may be used to translate specific mRNAs. However, it is unknown to what extent structural heterogeneity reflects genuine functional specialization rather than stochastic variations in ribosome assembly. Here, we address this question by combining cryo-electron microscopy and tomography to observe individual structurally heterogeneous ribosomes in bacterial cells. We show that 70% of ribosomes in Psychrobacter urativorans contain a second copy of the ribosomal protein bS20 at a previously unknown binding site on the large ribosomal subunit. We then determine that this second bS20 copy appears to be functionally neutral. This demonstrates that ribosome heterogeneity does not necessarily lead to functional specialization, even when it involves significant variations such as the presence or absence of a ribosomal protein. Instead, we show that heterogeneous ribosomes can cooperate in general protein synthesis rather than specialize in translating discrete populations of mRNA.

PubMed: 40113756
DOI: 10.1038/s41467-025-57955-8

実験手法

ELECTRON MICROSCOPY (3.2 Å)