9H6E

Complex of Histidine-containing phosphotransfer 1 (AHP1) and Response regulator 1 (ARR1) from A. thaliana

9H6E の概要

• エントリーDOI: 10.2210/pdb9h6e/pdb
• 分子名称: Histidine-containing phosphotransfer protein 1, Two-component response regulator ARR1, OXAMIC ACID, ... (6 entities in total)
• 機能のキーワード: response regulator, histidine-containing phosphotransfer, cytokinin signaling, protein-protein complex, signaling protein
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98833.93

構造登録者

Tran, L.H.,Ruszkowski, M. (登録日: 2024-10-24, 公開日: 2025-03-26)

主引用文献

Tran, L.H.,Ruszkowski, M.
ARR1 and AHP interactions in the multi-step phosphorelay system.
Front Plant Sci, 16:1537021-1537021, 2025

PubMed Abstract: Plants use multi-step phosphorelay (MSP) systems in response to exogenous and endogenous stimuli. Cytokinin and ethylene are among the factors that engage MSP signaling cascades but examples independent of phytohormones also exist. The MSP signaling involves four consecutive phosphorylation events at: (i) the kinase domain of the sensory histidine kinase, (ii) the receiver domain of the latter protein, (iii) the histidine-containing phosphotransfer protein, and (iv) the response regulator. In , there are eight canonical histidine kinases, five histidine-containing phosphotransfer proteins (AHPs), one pseudo AHP, and 23 response regulators (ARRs). This redundancy suggests complex interactions between signaling pathways, including those involved in phytohormone cross-talk. To bring new insights at the molecular level, we investigated the structural and biophysical characteristics of the AHP1/ARR1 complex. ARR1, a type-B ARR, contains the GARP domain for DNA binding, in addition to the canonical receiver domain that mediates AHP1 interaction. We compared the ARR1 affinities across all five active AHPs and found a modest, two-fold higher affinity for AHP1. This result suggests that while ARR1 shows a slight preference for AHP1, it can also interact with AHP2-5, which potentially makes ARR1 a central node in signaling and a cross-talk modulator. In addition, we discuss the oligomerization state of AHP and related proteins utilizing all available experimental data to conclude that free AHPs are most likely monomeric.

PubMed: 40084109
DOI: 10.3389/fpls.2025.1537021

実験手法

X-RAY DIFFRACTION (2.87 Å)