9H4A
Ribonuclease-three Like 4 crystallization and structure determination at room temperature in the CrystalChip
Summary for 9H4A
| Entry DOI | 10.2210/pdb9h4a/pdb |
| Descriptor | Protein NUCLEAR FUSION DEFECTIVE 2 (1 entity in total) |
| Functional Keywords | ribonuclease-three like, microfluidics, room temperature, rtl4, rna binding protein |
| Biological source | Arabidopsis thaliana (thale cress) |
| Total number of polymer chains | 2 |
| Total formula weight | 35218.36 |
| Authors | Engilberge, S.,Vincent, R.,Coudray, L.,Nilles, L.,Scheer, H.,Ritzenthaler, C.,Sauter, C. (deposition date: 2024-10-18, release date: 2024-12-11, Last modification date: 2025-04-16) |
| Primary citation | Pachl, P.,Coudray, L.,Vincent, R.,Nilles, L.,Scheer, H.,Ritzenthaler, C.,Fejfarova, A.,Rezacova, P.,Engilberge, S.,Sauter, C. Protein crystallization and structure determination at room temperature in the CrystalChip. Febs Open Bio, 15:532-541, 2025 Cited by PubMed Abstract: The production of high-quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties. This protocol describes the implementation of a simple and user-friendly microfluidic device that allows both the production of crystals by the counter-diffusion method and their in situ analysis by serial crystallography. As an illustration, the whole procedure is used to determine the crystal structure of three proteins from data collected at room temperature at a synchrotron radiation source. PubMed: 39572886DOI: 10.1002/2211-5463.13932 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.8 Å) |
Structure validation
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