9GVA

Crystal structure of the gamma carbonic anhydrase from Porphyromonas gingivalis

これはPDB形式変換不可エントリーです。

9GVA の概要

• エントリーDOI: 10.2210/pdb9gva/pdb
• 分子名称: Hexapeptide transferase family protein, ZINC ION (3 entities in total)
• 機能のキーワード: carbonic anhydrase gamma, porphyromonas gingivalis, metalloenzyme, bacteria, lyase
• 由来する生物種: Porphyromonas gingivalis
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 62748.05

構造登録者

Angeli, A.,Ferraroni, M. (登録日: 2024-09-23, 公開日: 2024-12-25)

主引用文献

Ferraroni, M.,Angeli, A.,De Luca, V.,Capasso, C.,Supuran, C.T.
Kinetic and structural studies of gamma-carbonic anhydrase from the oral pathogen Porphyromonas gingivalis.
J.Struct.Biol., 217:108154-108154, 2024

PubMed Abstract: Porphyromonas gingivalis, a key pathogen in periodontal, plays a critical role in systemic pathologiesdiseases by evading host defence mechanisms and invading periodontal tissues. Targeting its virulence mechanisms and overcoming drug resistance are essential steps toward effective therapeutic development. In this study, we focused on the Carbonic Anhydrase (CA, EC: 4.2.1.1) encoded by P. gingivalis as a potential drug target. We determined the crystal structure of PgiCA γ at a resolution of 2.4 Å and conducted kinetic characterization. The structure revealed that active PgiCA γ forms a trimer, with each monomer comprising a left-handed β-helix capped by a C-terminal α-helix and coordinated to a catalytic zinc ion through three histidine residues. Interestingly, one monomer displayed an atypical α-helix conformation, likely due to close interactions with neighbouring trimers within the crystal lattice (a probable crystallographic artefact). These findings provide new insights into the structural and functional properties of PgiCA γ, emphasizing its potential as a target for the development of novel anti-virulence therapies against P. gingivalis.

PubMed: 39647519
DOI: 10.1016/j.jsb.2024.108154

実験手法

X-RAY DIFFRACTION (2.4 Å)