9GK2

Surface-layer (S-layer) PS2 protein from Corynebacterium glutamicum

9GK2 の概要

• エントリーDOI: 10.2210/pdb9gk2/pdb
• EMDBエントリー: 51414
• 分子名称: PS2 (1 entity in total)
• 機能のキーワード: s-layer, corynebacterium, surface, structural protein
• 由来する生物種: Corynebacterium glutamicum
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 858003.46

構造登録者

Sogues, A.,Remaut, H.,Sleutel, M. (登録日: 2024-08-23, 公開日: 2024-10-02, 最終更新日: 2026-03-04)

主引用文献

Sogues, A.,Sleutel, M.,Petit, J.,Megrian, D.,Bayan, N.,Wehenkel, A.M.,Remaut, H.
Cryo-EM structure and polar assembly of the PS2 S-layer of Corynebacterium glutamicum.
Proc.Natl.Acad.Sci.USA, 122:e2426928122-e2426928122, 2025

PubMed Abstract: The polar-growing have a complex cell envelope architecture characterized by the presence of a specialized outer membrane composed of mycolic acids. In some , this mycomembrane is further supported by a proteinaceous surface layer or "S-layer," whose function, structure, and mode of assembly remain largely enigmatic. Here, we isolated ex vivo PS2 S-layers from the industrially important and determined its atomic structure by 3D cryo-EM reconstruction. PS2 monomers consist of a six-helix bundle "core," a three-helix bundle "arm," and a C-terminal transmembrane (TM) helix. The PS2 core oligomerizes into hexameric units anchored in the mycomembrane by a channel-like coiled-coil of the TM helices. The PS2 arms mediate trimeric lattice contacts, crystallizing the hexameric units into an intricate semipermeable lattice. Using pulse-chase live cell imaging, we show that the PS2 lattice is incorporated at the poles, coincident with the actinobacterial elongasome. Finally, phylogenetic analysis shows a paraphyletic distribution and dispersed chromosomal location of PS2 in as a result of multiple recombination events and losses. These findings expand our understanding of S-layer biology and enable applications of membrane-supported self-assembling bioengineered materials.

PubMed: 40729392
DOI: 10.1073/pnas.2426928122

実験手法

ELECTRON MICROSCOPY (2.5 Å)