9G97

Lipid III flippase WzxE with NB10 nanobody in outward-facing conformation at 0.9688 A

9G97 の概要

• エントリーDOI: 10.2210/pdb9g97/pdb
• 関連するPDBエントリー: 9G95
• 分子名称: Lipid III flippase, NB10 Nanobody, [(Z)-octadec-9-enyl] (2R)-2,3-bis(oxidanyl)propanoate, ... (9 entities in total)
• 機能のキーワード: membrane protein, flippase, cell wall, enterobacterial common antigen, lipid iii, transport protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65356.08

構造登録者

Le Bas, A.,El Omari, K.,Lee, M.,Naismith, J.H. (登録日: 2024-07-24, 公開日: 2025-01-22)

主引用文献

Le Bas, A.,Clarke, B.R.,Teelucksingh, T.,Lee, M.,El Omari, K.,Giltrap, A.M.,McMahon, S.A.,Liu, H.,Beale, J.H.,Mykhaylyk, V.,Duman, R.,Paterson, N.G.,Ward, P.N.,Harrison, P.J.,Weckener, M.,Pardon, E.,Steyaert, J.,Liu, H.,Quigley, A.,Davis, B.G.,Wagner, A.,Whitfield, C.,Naismith, J.H.
Structure of WzxE the lipid III flippase for Enterobacterial Common Antigen polysaccharide.
Open Biology, 15:240310-240310, 2025

PubMed Abstract: The enterobacterial common antigen (ECA) is conserved in Gram-negative bacteria of the order although its function is debated. ECA biogenesis depends on the Wzx/Wzy-dependent strategy whereby the newly synthesized lipid-linked repeat units, lipid III, are transferred across the inner membrane by the lipid III flippase WzxE. WzxE is part of the Wzx family and required in many glycan assembly systems, but an understanding of its molecular mechanism is hindered due to a lack of structural evidence. Here, we present the first X-ray structures of WzxE from in complex with nanobodies. Both inward- and outward-facing conformations highlight two pairs of arginine residues that move in a reciprocal fashion, enabling flipping. One of the arginine pairs coordinated to a glutamate residue is essential for activity along with the C-terminal arginine rich tail located close to the entrance of the lumen. This work helps understand the translocation mechanism of the Wzx flippase family.

PubMed: 39772807
DOI: 10.1098/rsob.240310

実験手法

X-RAY DIFFRACTION (2.31 Å)