9G08

Structure of human RNF213 bound to the secreted effector IpaH1.4 from Shigella flexneri

9G08 の概要

• エントリーDOI: 10.2210/pdb9g08/pdb
• EMDBエントリー: 50913
• 分子名称: E3 ubiquitin-protein ligase RNF213, E3 ubiquitin-protein ligase IpaH1.4, ADENOSINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: rnf213, ipah1.4, ipah, e3 ligase, ring, lrr, nel, secreted effector, shigella, inhibitor, complex, aaa atpase, antimicrobial protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 662103.06

構造登録者

Naydenova, K.,Randow, F. (登録日: 2024-07-07, 公開日: 2025-04-16, 最終更新日: 2025-10-01)

主引用文献

Naydenova, K.,Boyle, K.B.,Pathe, C.,Pothukuchi, P.,Crespillo-Casado, A.,Scharte, F.,Hammoudi, P.M.,Otten, E.G.,Alto, N.M.,Randow, F.
Shigella flexneri evades LPS ubiquitylation through IpaH1.4-mediated degradation of RNF213.
Nat.Struct.Mol.Biol., 32:1741-1751, 2025

PubMed Abstract: Pathogens have evolved diverse strategies to counteract host immunity. Ubiquitylation of lipopolysaccharide (LPS) on cytosol-invading bacteria by the E3 ligase RNF213 creates 'eat me' signals for antibacterial autophagy, but whether and how cytosol-adapted bacteria avoid LPS ubiquitylation remains poorly understood. Here, we show that the enterobacterium Shigella flexneri actively antagonizes LPS ubiquitylation through IpaH1.4, a secreted effector protein with ubiquitin E3 ligase activity. IpaH1.4 binds to RNF213, ubiquitylates it and targets it for proteasomal degradation, thus counteracting host-protective LPS ubiquitylation. To understand how IpaH1.4 recognizes RNF213, we determined the cryogenic electron microscopy structure of the IpaH1.4-RNF213 complex. The specificity of the interaction is achieved through the leucine-rich repeat of IpaH1.4, which binds the RING domain of RNF213 by hijacking the conserved RING interface required for binding to ubiquitin-charged E2 enzymes. IpaH1.4 also targets other E3 ligases involved in inflammation and immunity through binding to the E2-interacting face of their RING domains, including the E3 ligase LUBAC that is required for the synthesis of M1-linked ubiquitin chains on cytosol-invading bacteria downstream of RNF213. We conclude that IpaH1.4 has evolved to antagonize multiple antibacterial and proinflammatory host E3 ligases.

PubMed: 40205224
DOI: 10.1038/s41594-025-01530-8

実験手法

ELECTRON MICROSCOPY (3.3 Å)