9G06 の概要
| エントリーDOI | 10.2210/pdb9g06/pdb |
| EMDBエントリー | 50912 |
| 分子名称 | Small ribosomal subunit protein uS3, Small ribosomal subunit protein uS13, Small ribosomal subunit protein uS14, ... (28 entities in total) |
| 機能のキーワード | antibiotics, 30s initiation, translational inhibitor, translation |
| 由来する生物種 | Escherichia coli 詳細 |
| タンパク質・核酸の鎖数 | 24 |
| 化学式量合計 | 835762.97 |
| 構造登録者 | |
| 主引用文献 | Safdari, H.A.,Morici, M.,Sanchez-Castro, A.,Dallape, A.,Paternoga, H.,Giuliodori, A.M.,Fabbretti, A.,Milon, P.,Wilson, D.N. The translation inhibitors kasugamycin, edeine and GE81112 target distinct steps during 30S initiation complex formation. Nat Commun, 16:2470-2470, 2025 Cited by PubMed Abstract: During bacterial translation initiation, the 30S ribosomal subunit, initiation factors, and initiator tRNA define the reading frame of the mRNA. This process is inhibited by kasugamycin, edeine and GE81112, however, their mechanisms of action have not been fully elucidated. Here we present cryo-electron microscopy structures of 30S initiation intermediate complexes formed in the presence of kasugamycin, edeine and GE81112 at resolutions of 2.0-2.9 Å. The structures reveal that all three antibiotics bind within the E-site of the 30S and preclude 30S initiation complex formation. While kasugamycin and edeine affect early steps of 30S pre-initiation complex formation, GE81112 stalls pre-initiation complex formation at a further step by allowing start codon recognition, but impeding IF3 departure. Collectively, our work highlights how chemically distinct compounds binding at a conserved site on the 30S can interfere with translation initiation in a unique manner. PubMed: 40075065DOI: 10.1038/s41467-025-57731-8 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (2.85 Å) |
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