9FTL

Asgard ESCRT-IIIB filament structure

9FTL の概要

• エントリーDOI: 10.2210/pdb9ftl/pdb
• EMDBエントリー: 50748
• 分子名称: Heimdallarchaeota archaeon AB_125 ESCRT-IIIB (1 entity in total)
• 機能のキーワード: escrt-iii, filament, membrane binding protein, membrane remodelling, asgard archaea, membrane protein
• 由来する生物種: Candidatus Heimdallarchaeota archaeon
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 48260.54

構造登録者

Chaaban, S.,Souza, D.P.,Baum, B. (登録日: 2024-06-24, 公開日: 2025-04-16)

主引用文献

Souza, D.P.,Espadas, J.,Chaaban, S.,Moody, E.R.R.,Hatano, T.,Balasubramanian, M.,Williams, T.A.,Roux, A.,Baum, B.
Asgard archaea reveal the conserved principles of ESCRT-III membrane remodeling.
Sci Adv, 11:eads5255-eads5255, 2025

PubMed Abstract: ESCRT-III proteins assemble into composite polymers that undergo stepwise changes in composition and structure to deform membranes across the tree of life. Here, using a phylogenetic analysis, we demonstrate that the two endosomal sorting complex required for transport III (ESCRT-III) proteins present in eukaryote's closest Asgard archaeal relatives are evolutionarily related to the B- and A-type eukaryotic paralogs that initiate and execute membrane remodeling, respectively. We show that Asgard ESCRT-IIIB assembles into parallel arrays on planar membranes to initiate membrane deformation, from where it recruits ESCRT-IIIA to generate composite polymers. Last, we show that Asgard ESCRT-IIIA is able to remodel membranes into tubes as a likely prelude to scission. Together, these data reveal a set of conserved principles governing ESCRT-III-dependent membrane remodeling that first emerged in a two-component ESCRT-III system in archaea.

PubMed: 39919172
DOI: 10.1126/sciadv.ads5255

実験手法

ELECTRON MICROSCOPY (2.91 Å)