9FSA

Cell wall anchoring domain of the surface layer protein of Methanococcus voltae (aa 24-75; 484-576)

9FSA の概要

• エントリーDOI: 10.2210/pdb9fsa/pdb
• 分子名称: S-layer protein (2 entities in total)
• 機能のキーワード: surface layer protein, self assembly, cell wall binding, anchoring, symmetry, structural protein
• 由来する生物種: Methanococcus voltae; 詳細
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15757.84

構造登録者

Grininger, C.,Sagmeister, T.,Pavkov-Keller, T. (登録日: 2024-06-20, 公開日: 2024-08-28, 最終更新日: 2024-10-02)

主引用文献

Buhlheller, C.,Sagmeister, T.,Grininger, C.,Gubensak, N.,Sleytr, U.B.,Uson, I.,Pavkov-Keller, T.
SymProFold: Structural prediction of symmetrical biological assemblies.
Nat Commun, 15:8152-8152, 2024

PubMed Abstract: Symmetry in nature often emerges from self-assembly processes and serves a wide range of functions. Cell surface layers (S-layers) form symmetrical lattices on many bacterial and archaeal cells, playing essential roles such as facilitating cell adhesion, evading the immune system, and protecting against environmental stress. However, the experimental structural characterization of these S-layers is challenging due to their self-assembly properties and high sequence variability. In this study, we introduce the SymProFold pipeline, which utilizes the high accuracy of AlphaFold-Multimer predictions to derive symmetrical assemblies from protein sequences, specifically focusing on two-dimensional S-layer arrays and spherical viral capsids. The pipeline tests all known symmetry operations observed in these systems (p1, p2, p3, p4, and p6) and identifies the most likely symmetry for the assembly. The predicted models were validated using available experimental data at the cellular level, and additional crystal structures were obtained to confirm the symmetry and interfaces of several SymProFold assemblies. Overall, the SymProFold pipeline enables the determination of symmetric protein assemblies linked to critical functions, thereby opening possibilities for exploring functionalities and designing targeted applications in diverse fields such as nanotechnology, biotechnology, medicine, and materials and environmental sciences.

PubMed: 39294115
DOI: 10.1038/s41467-024-52138-3

実験手法

X-RAY DIFFRACTION (2.05 Å)