9FQT

Cryo-EM structure of MmCAT1 bound with FrMLV-RBD in the apo inward-open state

9FQT の概要

• エントリーDOI: 10.2210/pdb9fqt/pdb
• EMDBエントリー: 50668
• 分子名称: High affinity cationic amino acid transporter 1,Green fluorescent protein, Surface protein, alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (6 entities in total)
• 機能のキーワード: mmcat1, frmlv-rbd, slc, viral tropism, membrane protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 131427.40

構造登録者

Ye, M.,Zhou, D.,Pike, A.C.W.,Wang, S.,Wang, D.,Bakshi, S.,Brooke, L.,Williams, E.,Elkins, J.,Stuart, D.I.,Sauer, D.B. (登録日: 2024-06-17, 公開日: 2025-07-02, 最終更新日: 2026-04-08)

主引用文献

Ye, M.,Liang, Z.,Zhou, D.,Pike, A.C.W.,Wang, S.,Wang, D.,Bakshi, S.,Brooke, L.,Williams, E.P.,Elkins, J.M.,Kessler, B.M.,Stuart, D.I.,Sauer, D.B.
Amino acid and viral binding by the high-affinity Cationic Amino acid Transporter 1 (CAT1) from Mus musculus.
Nat Commun, 17:-, 2026

PubMed Abstract: Arginine, lysine, and ornithine are critical to several fundamental aspects of organismal physiology, including protein structure and function, the urea cycle, and intracellular signaling. These cationic amino acids are imported by several membrane transporters, most notably the Cationic Amino acid Transporters (CATs) in the SLC7 family. Of these, CAT1 is also the receptor for two orthoretroviruses, and determines the host tropism for these viruses. Here, using a combination of CryoEM and in vitro biochemical techniques, we characterize the substrate recognition and transport of CAT1 from Mus musculus. Further, by determining the structures of MmCAT1 in complex with the receptor binding domain from the Friend Murine Leukemia Virus, we identify the key structural interactions that determine the virus' rodent-specific tropism.

PubMed: 41698924
DOI: 10.1038/s41467-026-69421-0

実験手法

ELECTRON MICROSCOPY (3.5 Å)