9FOW

GPR180 N-terminal domain

9FOW の概要

• エントリーDOI: 10.2210/pdb9fow/pdb
• 分子名称: Integral membrane protein GPR180, alpha-L-fucopyranose-(1-3)-[2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-3)][alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: gold, gost, gpr180, unknown function
• 由来する生物種: Mus musculus (house mouse)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 51370.62

構造登録者

Mitrovic, S.A.,Reindl, S.,Nar, H. (登録日: 2024-06-12, 公開日: 2024-10-16, 最終更新日: 2024-12-11)

主引用文献

Mitrovic, S.A.,Demalgiriya-Gamage, C.,Winter, L.M.,Kiechle, T.,Ebenhoch, R.,Neubauer, H.,Stierstorfer, B.,Frego, L.,Wolfrum, C.,Reindl, S.,Nar, H.
GPR180 is a new member of the Golgi-dynamics domain seven-transmembrane helix protein family.
Commun Biol, 7:1588-1588, 2024

PubMed Abstract: GOLD domain seven-transmembrane helix (GOST) proteins form a new protein family involved in trafficking of membrane-associated cargo. They share a characteristic extracellular/luminal Golgi-dynamics (GOLD) domain, possibly responsible for ligand recognition. Based on structural homology, GPR180 is a new member of this protein family, but little is known about the cellular role of GPR180. Here we show the X-ray structure of the N-terminal domain of GPR180 (1.9 Å) and can confirm the homology to GOLD domains. Using cellular imaging we show the localization of GPR180 in intracellular vesicular structures implying its exposure to acidic pH environments. With Hydrogen/Deuterium Exchange-Mass Spectrometry (HDX-MS) we identify pH-dependent conformational changes, which can be mapped to a putative ligand binding site in the transmembrane region. The results reveal GPR180's role in intracellular vesicles and offer insights into the pH-dependent function of this conserved GOST protein.

PubMed: 39609618
DOI: 10.1038/s42003-024-07260-9

実験手法

X-RAY DIFFRACTION (1.878 Å)