9FN1

360 A Loki CHMP4-7 rods

9FN1 の概要

• エントリーDOI: 10.2210/pdb9fn1/pdb
• EMDBエントリー: 50583
• 分子名称: Vps20/32/60-like protein (ESCRT-III) (1 entity in total)
• 機能のキーワード: escrt-iii, membrane remodling, tubulation, archaea, loki, lipid binding protein
• 由来する生物種: Candidatus Lokiarchaeia archaeon
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 24739.96

構造登録者

Melnikov, N.,Junglas, B.,Halbi, G.,Nachmias, D.,Upcher, A.,Zalk, R.,Sachse, C.,Bernheim-Groswasser, A.,Elia, N. (登録日: 2024-06-07, 公開日: 2025-04-16, 最終更新日: 2025-10-29)

主引用文献

Melnikov, N.,Junglas, B.,Halbi, G.,Nachmias, D.,Zerbib, E.,Gueta, N.,Upcher, A.,Zalk, R.,Sachse, C.,Bernheim-Groswasser, A.,Elia, N.
The Asgard archaeal ESCRT-III system forms helical filaments and remodels eukaryotic-like membranes.
Embo J., 44:665-681, 2025

PubMed Abstract: The ESCRT machinery mediates membrane remodeling in numerous processes in cells including cell division and nuclear membrane reformation. The identification of ESCRT homologs in Asgard archaea, currently considered the closest prokaryotic relative of eukaryotes, implies a role for ESCRTs in the membrane remodeling processes that occurred during eukaryogenesis. Yet, the function of these distant ESCRT homologs is mostly unresolved. Here we show that Asgard ESCRT-III proteins of the Lokiarcheota self-assemble into helical filaments, a hallmark of the ESCRT system. We determined the cryo-EM structure of the filaments at 3.6 Å resolution and found that they share features of bacterial and eukaryotic ESCRT-III assemblies. Markedly, Asgard ESCRT-III filaments bound and deformed eukaryotic-like membrane vesicles. Oligonucleotides facilitated the assembly of ESCRT-III filaments and tuned the extent of membrane remodeling. The ability of Asgard archaeal ESCRTs to remodel eukaryotic-like membranes, which are fundamentally different from archaeal membranes, and the structural properties of these proteins places them at the junction between prokaryotes and eukaryotes.

PubMed: 39753954
DOI: 10.1038/s44318-024-00346-4

実験手法

ELECTRON MICROSCOPY (3.55 Å)