9FHP

CryoEM structure of wild-type Turnip Yellows Virus

9FHP の概要

• エントリーDOI: 10.2210/pdb9fhp/pdb
• 関連するPDBエントリー: 6rtk
• EMDBエントリー: 10003
• 分子名称: Minor capsid readthrough protein (1 entity in total)
• 機能のキーワード: read-through protein, virus
• 由来する生物種: Turnip yellows virus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 67567.56

構造登録者

Trapani, S.,Lai Kee Him, J.,Hoh, F.,Brault, V.,Bron, P. (登録日: 2024-05-28, 公開日: 2024-06-05, 最終更新日: 2025-05-21)

主引用文献

Lai-Kee-Him, J.,Trapani, S.,Boissinot, S.,Reinbold, C.,Fallet, C.,Ancelin, A.,Lecorre, F.,Hoh, F.,Ziegler-Graff, V.,Brault, V.,Bron, P.
Structure of the turnip yellows virus particles.
Virology, 607:110514-110514, 2025

PubMed Abstract: Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP∗) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP∗ contains the CP sequence fused with a structured domain, denoted RTD, which is a key determinant of virus transmission. Though both CP and RTP∗ are involved in virus movement and aphid transmission, how RTP∗ is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP∗ does not impair the capsid structure, and the RTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP∗s suggests a random insertion.

PubMed: 40179450
DOI: 10.1016/j.virol.2025.110514

実験手法

ELECTRON MICROSCOPY (4.08 Å)