9Q8J
CryoEM structure of modified Turnip Yellows Virus devoid of minor capsid protein readthrough domain
Replaces: 6RTKSummary for 9Q8J
| Entry DOI | 10.2210/pdb9q8j/pdb |
| Related | 9fhp |
| EMDB information | 10001 10003 |
| Descriptor | Minor capsid readthrough protein (1 entity in total) |
| Functional Keywords | virus |
| Biological source | Turnip yellows virus |
| Total number of polymer chains | 3 |
| Total formula weight | 67567.56 |
| Authors | Trapani, S.,Lai Kee Him, J.,Hoh, F.,Brault, V.,Bron, P. (deposition date: 2025-02-24, release date: 2025-05-07) |
| Primary citation | Lai-Kee-Him, J.,Trapani, S.,Boissinot, S.,Reinbold, C.,Fallet, C.,Ancelin, A.,Lecorre, F.,Hoh, F.,Ziegler-Graff, V.,Brault, V.,Bron, P. Structure of the turnip yellows virus particles. Virology, 607:110514-110514, 2025 Cited by PubMed Abstract: Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP∗) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP∗ contains the CP sequence fused with a structured domain, denoted RTD, which is a key determinant of virus transmission. Though both CP and RTP∗ are involved in virus movement and aphid transmission, how RTP∗ is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP∗ does not impair the capsid structure, and the RTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP∗s suggests a random insertion. PubMed: 40179450DOI: 10.1016/j.virol.2025.110514 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.47 Å) |
Structure validation
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