9FFJ

Artificial metalloenzyme with a nickel-based 1,10-phenanthroline cofactor and streptavidin N49M-S112V mutant

これはPDB形式変換不可エントリーです。

9FFJ の概要

• エントリーDOI: 10.2210/pdb9ffj/pdb
• 分子名称: Streptavidin, 1,2-ETHANEDIOL, N-methyl-N-[(4,4,6,6-tetrahydroxy-4,6-dioxido-1,3,3a,5,6a-tetrahydrothien[3,4-d]imidazol-4-ium-2-yl)methyl]-5-(2,4,4-trihydroxy-2-keto-3,3a,5,6-tetrahydro-1H-thien[3,4-d]imidazol-4-ium-6-yl, ... (7 entities in total)
• 機能のキーワード: artificial metalloenzyme, nickel, metal binding protein
• 由来する生物種: Streptomyces avidinii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17718.99

構造登録者

Lau, K.,Wang, W.,Pojer, F.,Larabi, A. (登録日: 2024-05-23, 公開日: 2025-01-15, 最終更新日: 2025-02-26)

主引用文献

Wang, W.,Tachibana, R.,Zhang, K.,Lau, K.,Pojer, F.,Ward, T.R.,Hu, X.
Artificial Metalloenzymes with Two Catalytic Cofactors for Tandem Abiotic Transformations.
Angew.Chem.Int.Ed.Engl., 64:e202422783-e202422783, 2025

PubMed Abstract: Artificial metalloenzymes (ArMs) enable the integration of abiotic cofactors within a native protein scaffold, allowing for non-natural catalytic activities. Previous ArMs, however, have primarily relied on single cofactor systems, limiting them to only one catalytic function. Here we present an approach to construct ArMs embedding two catalytic cofactors based on the biotin-streptavidin technology. By incorporating multiple catalytic cofactors into the four binding sites of streptavidin, we engineered programmable ArMs for tandem abiotic transformations including an enantioselective formal C-H hydroxylation and a photooxidation-Michael addition. This work thus outlines a promising strategy for the development of ArMs embedding multiple cofactors.

PubMed: 39760306
DOI: 10.1002/anie.202422783

実験手法

X-RAY DIFFRACTION (1.27 Å)