9F2T

Ferric-mycobactin receptor (FemA)

9F2T の概要

• エントリーDOI: 10.2210/pdb9f2t/pdb
• 分子名称: Ferric-mycobactin receptor, FemA, 1,2-ETHANEDIOL, ACETATE ION, ... (5 entities in total)
• 機能のキーワード: tonb-dependent transporter, membrane protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 170605.23

構造登録者

Moynie, L. (登録日: 2024-04-24, 公開日: 2025-02-26, 最終更新日: 2025-04-02)

主引用文献

Will, V.,Moynie, L.,Si Ahmed Charrier, E.,Le Bas, A.,Kuhn, L.,Volck, F.,Chicher, J.,Aksoy, H.,Madec, M.,Antheaume, C.,Mislin, G.L.A.,Schalk, I.J.
Structure of the Outer Membrane Transporter FemA and Its Role in the Uptake of Ferric Dihydro-Aeruginoic Acid and Ferric Aeruginoic Acid in Pseudomonas aeruginosa .
Acs Chem.Biol., 20:690-706, 2025

PubMed Abstract: Iron is essential for bacterial growth, and synthesizes the siderophores pyochelin (PCH) and pyoverdine to acquire it. PCH contains a thiazolidine ring that aids in iron chelation but is prone to hydrolysis, leading to the formation of 2-(2-hydroxylphenyl)-thiazole-4-carbaldehyde (IQS). Using mass spectrometry, we demonstrated that PCH undergoes hydrolysis and oxidation in solution, resulting in the formation of aeruginoic acid (AA). This study used proteomic analyses and fluorescent reporters to show that AA, dihydroaeruginoic acid (DHA), and PCH induce the expression of , a gene encoding the ferri-mycobactin outer membrane transporter in . Notably, the induction by AA and DHA was observed only in strains unable to produce pyoverdine, suggesting their weaker iron-chelating ability compared to that of pyoverdine. Fe uptake assays demonstrated that both AA-Fe and DHA-Fe complexes are transported via FemA; however, no uptake was observed for PCH-Fe through this transporter. Structural studies revealed that FemA is able to bind AA-Fe or DHA-Fe complexes. Key interactions are conserved between FemA and these two complexes, with specificity primarily driven by one of the two siderophore molecules. Interestingly, although no iron uptake was noted for PCH through FemA, the transporter also binds PCH-Fe in a similar manner. These findings show that under moderate iron deficiency, when only PCH is produced by , degradation products AA and DHA enhance iron uptake by inducing expression and facilitating iron transport through FemA. This provides new insights into the pathogen's strategies for iron homeostasis.

PubMed: 40035455
DOI: 10.1021/acschembio.4c00820

実験手法

X-RAY DIFFRACTION (2.2 Å)