9EVS の概要
| エントリーDOI | 10.2210/pdb9evs/pdb |
| EMDBエントリー | 50011 |
| 分子名称 | Small ribosomal subunit protein uS3m, uS12m, uS13m, ... (93 entities in total) |
| 機能のキーワード | mitochondria, trna, mitoribosome, ribosome, plant |
| 由来する生物種 | Brassica oleracea var. botrytis 詳細 |
| タンパク質・核酸の鎖数 | 88 |
| 化学式量合計 | 3686078.77 |
| 構造登録者 | |
| 主引用文献 | Skaltsogiannis, V.,Wolff, P.,Nguyen, T.T.,Corre, N.,Pflieger, D.,Blevins, T.,Hashem, Y.,Giege, P.,Waltz, F. Structural insights into cauliflower mitoribosome in translation state and in association with a late assembly factor. Nat Commun, 16:10839-10839, 2025 Cited by PubMed Abstract: Ribosomes are key molecular machines that translate mRNA into proteins. Mitoribosomes are specific ribosomes found in mitochondria, which have been shown to be remarkably diverse across eukaryotic lineages. In plants, they possess unique features, including additional rRNA domains stabilized by plant-specific proteins. However, the structural specificities of plant mitoribosomes in translation state remained unknown. We used cryo-electron microscopy to provide a high-resolution structural characterization of the cauliflower mitoribosome, in translating and maturation states. The structure reveals the mitoribosome bound with a tRNA in the peptidyl site, along with a segment of mRNA and a nascent polypeptide. Moreover, using structural data, nanopore sequencing and mass spectrometry, we identify a set of 19 ribosomal RNA modifications. Additionally, we observe a late assembly intermediate of the small ribosomal subunit, in complex with the RsgA assembly factor. This reveals how a plant-specific extension of RsgA blocks the mRNA channel to prevent premature mRNA association before complete small subunit maturation. Our findings elucidate key aspects of translation in angiosperm plant mitochondria, revealing its distinct features compared to other eukaryotic lineages. PubMed: 41330901DOI: 10.1038/s41467-025-65864-z 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.05 Å) |
構造検証レポート
検証レポート(詳細版)
をダウンロード
をダウンロード
