9ETU

Archaellum filament from the Halobacterium salinarum deltaAgl27 strain

これはPDB形式変換不可エントリーです。

9ETU の概要

• エントリーDOI: 10.2210/pdb9etu/pdb
• EMDBエントリー: 19962
• 分子名称: Archaellin, 3-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-beta-D-glucopyranuronic acid-(1-4)-beta-D-glucopyranose (2 entities in total)
• 機能のキーワード: archaellum, haloarcheon, archaellin, structural protein
• 由来する生物種: Halobacterium salinarum
• タンパク質・核酸の鎖数: 25
• 化学式量合計: 533322.68

構造登録者

Grossman-Haham, I.,Shahar, A. (登録日: 2024-03-27, 公開日: 2024-07-17, 最終更新日: 2024-11-20)

主引用文献

Sofer, S.,Vershinin, Z.,Mashni, L.,Zalk, R.,Shahar, A.,Eichler, J.,Grossman-Haham, I.
Perturbed N-glycosylation of Halobacterium salinarum archaellum filaments leads to filament bundling and compromised cell motility.
Nat Commun, 15:5841-5841, 2024

PubMed Abstract: The swimming device of archaea-the archaellum-presents asparagine (N)-linked glycans. While N-glycosylation serves numerous roles in archaea, including enabling their survival in extreme environments, how this post-translational modification contributes to cell motility remains under-explored. Here, we report the cryo-EM structure of archaellum filaments from the haloarchaeon Halobacterium salinarum, where archaellins, the building blocks of the archaellum, are N-glycosylated, and the N-glycosylation pathway is well-resolved. We further determined structures of archaellum filaments from two N-glycosylation mutant strains that generate truncated glycans and analyzed their motility. While cells from the parent strain exhibited unidirectional motility, the N-glycosylation mutant strain cells swam in ever-changing directions within a limited area. Although these mutant strain cells presented archaellum filaments that were highly similar in architecture to those of the parent strain, N-linked glycan truncation greatly affected interactions between archaellum filaments, leading to dramatic clustering of both isolated and cell-attached filaments. We propose that the N-linked tetrasaccharides decorating archaellins act as physical spacers that minimize the archaellum filament aggregation that limits cell motility.

PubMed: 38992036
DOI: 10.1038/s41467-024-50277-1

実験手法

ELECTRON MICROSCOPY (3.33 Å)