9ETO

PsiK from Psilocybe cubensis

Summary for 9ETO

• Entry DOI: 10.2210/pdb9eto/pdb
• Descriptor: 4-hydroxytryptamine kinase, DI(HYDROXYETHYL)ETHER, SULFATE ION, ... (6 entities in total)
• Functional Keywords: kinase, transferase
• Biological source: Psilocybe cubensis
• Total number of polymer chains: 2
• Total formula weight: 83275.01

Authors

Werten, S.,Rupp, B. (deposition date: 2024-03-26, release date: 2024-11-06, Last modification date: 2025-02-19)

Primary citation

Rogge, K.,Wagner, T.J.,Hoffmeister, D.,Rupp, B.,Werten, S.
Substrate recognition by the 4-hydroxytryptamine kinase PsiK in psilocybin biosynthesis.
Febs Lett., 599:447-455, 2025

PubMed Abstract: Psilocybin, the natural hallucinogen from Psilocybe (magic) mushrooms, is a highly promising drug candidate for the treatment of depression and several other mental health conditions. Biosynthesis of psilocybin from the amino acid l-tryptophan involves four strictly sequential modifications. The third of these, ATP-dependent phosphorylation of the intermediate 4-hydroxytryptamine, is catalysed by PsiK. Here we present a crystallographic analysis and a structure-based mutagenesis study of this kinase, providing insight into its mode of substrate recognition. The results of our work will support future bioengineering efforts aimed at generating variants of psilocybin with enhanced therapeutic properties.

PubMed: 39449146
DOI: 10.1002/1873-3468.15042

Experimental method

X-RAY DIFFRACTION (2.54 Å)