9EOQ

Cryo-EM Structure of a 1033 Scaffold Base DNA Origami Nanostructure V4 and TBA

9EOQ の概要

• エントリーDOI: 10.2210/pdb9eoq/pdb
• EMDBエントリー: 19867
• 分子名称: DNA (42-MER), DNA (5'-D(P*AP*TP*AP*TP*AP*GP*CP*GP*TP*GP*GP*AP*AP*GP*T)-3') (2 entities in total)
• 機能のキーワード: dna origami, multilayer, square lattice, 1033 scaffold, dna, tba
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 17707.39

構造登録者

Ali, K.,Georg, K.,Volodymyr, M.,Johanna, G.,Maximilian, N.H.,Lukas, K.,Simone, C.,Hendrik, D. (登録日: 2024-03-15, 公開日: 2024-04-24, 最終更新日: 2024-05-08)

主引用文献

Khoshouei, A.,Kempf, G.,Mykhailiuk, V.,Griessing, J.M.,Honemann, M.N.,Kater, L.,Cavadini, S.,Dietz, H.
Designing Rigid DNA Origami Templates for Molecular Visualization Using Cryo-EM.
Nano Lett., 24:5031-5038, 2024

PubMed Abstract: DNA origami, a method for constructing nanostructures from DNA, offers potential for diverse scientific and technological applications due to its ability to integrate various molecular functionalities in a programmable manner. In this study, we examined the impact of internal crossover distribution and the compositional uniformity of staple strands on the structure of multilayer DNA origami using cryogenic electron microscopy (cryo-EM) single-particle analysis. A refined DNA object was utilized as an alignment framework in a host-guest model, where we successfully resolved an 8 kDa thrombin binding aptamer (TBA) linked to the host object. Our results broaden the spectrum of DNA in structural applications.

PubMed: 38602296
DOI: 10.1021/acs.nanolett.4c00915

実験手法

ELECTRON MICROSCOPY (7.5 Å)