9ENE

Human pseudouridine synthase 3 (PUS3 D118A mutant) and two tRNA-Arg

9ENE の概要

• エントリーDOI: 10.2210/pdb9ene/pdb
• EMDBエントリー: 16917 16926 19832
• 分子名称: tRNA pseudouridine(38/39) synthase, tRNA-Arg (2 entities in total)
• 機能のキーワード: rna modification, pseudouridylation, trna, homodimer, rna binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 160307.39

構造登録者

Lin, T.-Y.,Jezowski, J.,Glatt, S. (登録日: 2024-03-12, 公開日: 2024-07-10, 最終更新日: 2024-07-24)

主引用文献

Lin, T.Y.,Kleemann, L.,Jezowski, J.,Dobosz, D.,Rawski, M.,Indyka, P.,Wazny, G.,Mehta, R.,Chramiec-Glabik, A.,Koziej, L.,Ranff, T.,Fufezan, C.,Wawro, M.,Kochan, J.,Bereta, J.,Leidel, S.A.,Glatt, S.
The molecular basis of tRNA selectivity by human pseudouridine synthase 3.
Mol.Cell, 84:2472-2489.e8, 2024

PubMed Abstract: Pseudouridine (Ψ), the isomer of uridine, is ubiquitously found in RNA, including tRNA, rRNA, and mRNA. Human pseudouridine synthase 3 (PUS3) catalyzes pseudouridylation of position 38/39 in tRNAs. However, the molecular mechanisms by which it recognizes its RNA targets and achieves site specificity remain elusive. Here, we determine single-particle cryo-EM structures of PUS3 in its apo form and bound to three tRNAs, showing how the symmetric PUS3 homodimer recognizes tRNAs and positions the target uridine next to its active site. Structure-guided and patient-derived mutations validate our structural findings in complementary biochemical assays. Furthermore, we deleted PUS1 and PUS3 in HEK293 cells and mapped transcriptome-wide Ψ sites by Pseudo-seq. Although PUS1-dependent sites were detectable in tRNA and mRNA, we found no evidence that human PUS3 modifies mRNAs. Our work provides the molecular basis for PUS3-mediated tRNA modification in humans and explains how its tRNA modification activity is linked to intellectual disabilities.

PubMed: 38996458
DOI: 10.1016/j.molcel.2024.06.013

実験手法

ELECTRON MICROSCOPY (3.15 Å)