9EH2

RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-FACT nucleosome upstream

これはPDB形式変換不可エントリーです。

9EH2 の概要

• エントリーDOI: 10.2210/pdb9eh2/pdb
• EMDBエントリー: 48044
• 分子名称: DNA-directed RNA polymerase subunit, DNA-directed RNA polymerases I, II, and III subunit RPABC5, RNA polymerase II subunit J, ... (35 entities in total)
• 機能のキーワード: setd2, transcription, h3k36me3, transferase-rna-dna complex, transferase/rna/dna
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 35
• 化学式量合計: 1977967.13

構造登録者

Markert, J.,Farnung, L. (登録日: 2024-11-21, 公開日: 2024-12-18, 最終更新日: 2025-02-12)

主引用文献

Markert, J.W.,Soffers, J.H.,Farnung, L.
Structural basis of H3K36 trimethylation by SETD2 during chromatin transcription.
Science, 387:528-533, 2025

PubMed Abstract: During transcription, RNA polymerase II traverses through chromatin, and posttranslational modifications including histone methylations mark regions of active transcription. Histone protein H3 lysine 36 trimethylation (H3K36me3), which is established by the histone methyltransferase SET domain containing 2 (SETD2), suppresses cryptic transcription, regulates splicing, and serves as a binding site for transcription elongation factors. The mechanism by which the transcription machinery coordinates the deposition of H3K36me3 is not well understood. Here we provide cryo-electron microscopy structures of mammalian RNA polymerase II-DSIF-SPT6-PAF1c-TFIIS-IWS1-SETD2-nucleosome elongation complexes, revealing that the transcription machinery regulates H3K36me3 deposition by SETD2 on downstream and upstream nucleosomes. SPT6 binds the exposed H2A-H2B dimer during transcription, and the SPT6 death-like domain mediates an interaction with SETD2 bound to a nucleosome upstream of RNA polymerase II.

PubMed: 39666822
DOI: 10.1126/science.adn6319

実験手法

ELECTRON MICROSCOPY (3.1 Å)