9EF2

Cryo-EM structure of alpha5beta1 integrin in complex with NeoNectin candidate 2, open conformation

9EF2 の概要

• エントリーDOI: 10.2210/pdb9ef2/pdb
• 関連するPDBエントリー: 9CKV 9DIA
• EMDBエントリー: 47968
• 分子名称: Integrin alpha-5, Integrin beta-1, NeoNectin candidate 2, ... (9 entities in total)
• 機能のキーワード: a5b1, de novo, tissue regeneration, extracellular matrix protein, signaling protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 206457.76

構造登録者

Werther, R.,Nguyen, A.,Estrada Alamo, K.A.,Wang, X.,Campbell, M.G. (登録日: 2024-11-19, 公開日: 2025-06-18, 最終更新日: 2025-09-10)

主引用文献

Wang, X.,Guillem-Marti, J.,Kumar, S.,Lee, D.S.,Cabrerizo-Aguado, D.,Werther, R.,Alamo, K.A.E.,Zhao, Y.T.,Nguyen, A.,Kopyeva, I.,Huang, B.,Li, J.,Hao, Y.,Li, X.,Brizuela-Velasco, A.,Murray, A.,Gerben, S.,Roy, A.,DeForest, C.A.,Springer, T.,Ruohola-Baker, H.,Cooper, J.A.,Campbell, M.G.,Manero, J.M.,Ginebra, M.P.,Baker, D.
De Novo Design of Integrin alpha 5 beta 1 Modulating Proteins to Enhance Biomaterial Properties.
Adv Mater, 37:e2500872-e2500872, 2025

PubMed Abstract: Integrin α5β1 is crucial for cell attachment and migration in development and tissue regeneration, and α5β1 binding proteins can have considerable utility in regenerative medicine and next-generation therapeutics. We use computational protein design to create de novo α5β1-specific modulating miniprotein binders, called NeoNectins, that bind to and stabilize the open state of α5β1. When immobilized onto titanium surfaces and throughout 3D hydrogels, the NeoNectins outperform native fibronectin (FN) and RGD peptides in enhancing cell attachment and spreading, and NeoNectin-grafted titanium implants outperformed FN- and RGD-grafted implants in animal models in promoting tissue integration and bone growth. NeoNectins should be broadly applicable for tissue engineering and biomedicine.

PubMed: 40489013
DOI: 10.1002/adma.202500872

実験手法

ELECTRON MICROSCOPY (3.36 Å)