9DH2

Structure of Fab in complex with NKG2D extracellular domain

9DH2 の概要

• エントリーDOI: 10.2210/pdb9dh2/pdb
• 分子名称: Fab heavy chain, Fab light chain, NKG2-D type II integral membrane protein (3 entities in total)
• 機能のキーワード: agonist, fab, receptor, immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 251581.48

構造登録者

Fallon, D.,Huang, C.S. (登録日: 2024-09-03, 公開日: 2024-12-04, 最終更新日: 2024-12-18)

主引用文献

Fallon, D.,Huang, C.S.,Ma, J.,Morgan, C.,Zhou, Z.S.
Agonistic anti-NKG2D antibody structure reveals unique stoichiometry and epitope compared to natural ligands.
Mabs, 16:2433121-2433121, 2024

PubMed Abstract: Natural killer (NK) cells are effector cells of the innate immune system that distinguish between healthy and abnormal cells through activating and inhibitory receptor signaling. NKG2D, a homodimeric activating receptor expressed on NK cells, recognizes a diverse class of stress ligands expressed by cells experiencing infection, malignant transformation, chronic inflammation, and other cellular stresses. Despite the variety of NKG2D ligands, they all bind the receptor asymmetrically in a 1:1 ligand to homodimeric NKG2D stoichiometry. In contrast, as we report herein, the agonistic antibody 2D3 binds NKG2D with a 2:1 stoichiometry of its antigen binding fragments to homodimeric NKG2D and a largely distinct epitope. This binding interaction, as compared to NKG2D natural ligands, suggests there may be unique mechanisms to engage this receptor while offering possible benefits when incorporated into an IgG-based therapeutic.

PubMed: 39582357
DOI: 10.1080/19420862.2024.2433121

実験手法

X-RAY DIFFRACTION (2.98 Å)