9DCF

Structure of Coxsackievirus B3 cloverleaf RNA in complex with 3Cpro dimer

9DCF の概要

• エントリーDOI: 10.2210/pdb9dcf/pdb
• 分子名称: Protease 3C, cloverleaf RNA (3 entities in total)
• 機能のキーワード: enterovirus 3cpro, cloverleaf rna, coxsackievirus, protein-rna complex, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Coxsackievirus B3 (strain Nancy); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 71724.19

構造登録者

Gottipati, K.,Dias, D.S.A.N.,Choi, K.H. (登録日: 2024-08-26, 公開日: 2025-03-26)

主引用文献

Dias-Solange, D.,Le, M.T.,Gottipati, K.,Choi, K.H.
Structure of coxsackievirus cloverleaf RNA and 3C pro dimer establishes the RNA-binding mechanism of enterovirus protease 3C pro.
Sci Adv, 11:eads6862-eads6862, 2025

PubMed Abstract: In positive-strand RNA viruses, the genome serves as a template for both protein translation and negative-strand RNA synthesis. Enteroviruses use the cloverleaf RNA structure at the 5' end of the genome to balance these two processes. Cloverleaf acts as a promoter for RNA synthesis and forms a complex with viral 3CD protein, the precursor to 3C protease, and 3D polymerase. The interaction between cloverleaf and 3CD is mediated by the 3C domain, yet how 3C promotes specific RNA-binding is not clear. We report the structure of coxsackievirus cloverleaf RNA-3C complex, wherein two 3C molecules interact with cloverleaf stem-loop D. 3C dimer mainly recognizes the shape of the dsRNA helix through symmetric interactions, suggesting that 3C is a previously undiscovered type of RNA binding protein. We show that 3CD protein also dimerizes on cloverleaf RNA and binds the RNA with higher affinity than 3C. The structure provides insight into the RNA-binding mechanism of 3C or 3CD with other cis-acting replication elements.

PubMed: 40073119
DOI: 10.1126/sciadv.ads6862

実験手法

X-RAY DIFFRACTION (2.87 Å)