9DA9

Crystal structure of GluN1/GluN2A agonist-binding domains in complex with 7CKA and glutamate

9DA9 の概要

• エントリーDOI: 10.2210/pdb9da9/pdb
• 分子名称: Glutamate receptor ionotropic, NMDA 1, Glutamate receptor ionotropic, NMDA 2A, 7-Chlorokynurenic acid, ... (5 entities in total)
• 機能のキーワード: glutamate receptor ligand-gated ion channel neurotransmitter receptor synaptic transmission neuropharmacology, signaling protein
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 65496.07

構造登録者

Bosco, J.,Yates-Hansen, C.K.,McClelland, L.J.,Voronina, E.,Hansen, K.B. (登録日: 2024-08-22, 公開日: 2025-03-12, 最終更新日: 2025-04-16)

主引用文献

Bosco, J.,Gagliano, E.,Boshae, K.L.,Statz, J.P.,Wheeler, T.B.,Cuello, D.,Sliter, A.,Newby, C.,Lin, B.,Demeler, A.,Pierpont, C.L.,Yates-Hansen, C.,Sydor, M.J.,Ferrini, M.E.,Kuch, K.C.,Cooper, B.S.,Piggott, B.J.,Certel, S.J.,Hansen, K.B.,Sprang, S.R.,Bowler, B.,McClelland, L.,Berkmen, M.,Voronina, E.
A galactose-based auto-expression system improves T7-inducible protein production in Escherichia coli.
Sci Rep, 15:8936-8936, 2025

PubMed Abstract: Protein production using Escherichia coli is a cornerstone of modern biotechnology. In this study, we developed a novel auto-expression medium to maximize protein production. Each E. coli strain tested was capable of auto-expression in response to galactose, including strains in which the endogenous lacZ had been disrupted. This provides key evidence that galactose can regulate the lac operon independent of known lac operon-regulated metabolism. The enhanced capabilities of the novel auto-expression medium were documented across protein production systems including (1) increased yields for routinely expressed proteins (e.g. eGFP), (2) improved expression of human cytochrome c within a dual expression system, (3) robust auto-expression in lacZ-deficient strains producing proteins with challenging disulfide bonds, and (4) reproducible 8-fold increase in SpCas9 yields, at ≥ 95% purity. This novel medium can streamline production and improve yields for routine as well as challenging proteins, accelerating recombinant protein production and creating new opportunities in biotechnology and structural biology.

PubMed: 40089537
DOI: 10.1038/s41598-025-91954-5

実験手法

X-RAY DIFFRACTION (2.05 Å)