9D3E の概要
| エントリーDOI | 10.2210/pdb9d3e/pdb |
| EMDBエントリー | 46533 |
| 分子名称 | Human CCR6, anti-BRIL Fab Heavy chain, anti-BRIL Fab Nanobody, ... (7 entities in total) |
| 機能のキーワード | gpcr, antagonist, ccr6, bril, membrane protein |
| 由来する生物種 | Homo sapiens 詳細 |
| タンパク質・核酸の鎖数 | 4 |
| 化学式量合計 | 125775.21 |
| 構造登録者 | |
| 主引用文献 | Wasilko, D.J.,Gerstenberger, B.S.,Farley, K.A.,Li, W.,Alley, J.,Schnute, M.E.,Unwalla, R.J.,Victorino, J.,Crouse, K.K.,Ding, R.,Sahasrabudhe, P.V.,Vincent, F.,Frisbie, R.K.,Dermenci, A.,Flick, A.,Choi, C.,Chinigo, G.,Mousseau, J.J.,Trujillo, J.I.,Nuhant, P.,Mondal, P.,Lombardo, V.,Lamb, D.,Hogan, B.J.,Minhas, G.S.,Segala, E.,Oswald, C.,Windsor, I.W.,Han, S.,Rappas, M.,Cooke, R.M.,Calabrese, M.F.,Berstein, G.,Thorarensen, A.,Wu, H. Structural basis for CCR6 modulation by allosteric antagonists. Nat Commun, 15:7574-7574, 2024 Cited by PubMed Abstract: The CC chemokine receptor 6 (CCR6) is a potential target for chronic inflammatory diseases. Previously, we reported an active CCR6 structure in complex with its cognate chemokine CCL20, revealing the molecular basis of CCR6 activation. Here, we present two inactive CCR6 structures in ternary complexes with different allosteric antagonists, CCR6/SQA1/OXM1 and CCR6/SQA1/OXM2. The oxomorpholine analogues, OXM1 and OXM2 are highly selective CCR6 antagonists which bind to an extracellular pocket and disrupt the receptor activation network. An energetically favoured U-shaped conformation in solution that resembles the bound form is observed for the active analogues. SQA1 is a squaramide derivative with close-in analogues reported as antagonists of chemokine receptors including CCR6. SQA1 binds to an intracellular pocket which overlaps with the G protein site, stabilizing a closed pocket that is a hallmark of inactive GPCRs. Minimal communication between the two allosteric pockets is observed, in contrast to the prevalent allosteric cooperativity model of GPCRs. This work highlights the versatility of GPCR antagonism by small molecules, complementing previous knowledge of CCR6 activation, and sheds light on drug discovery targeting CCR6. PubMed: 39217154DOI: 10.1038/s41467-024-52045-7 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (3.02 Å) |
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