9CWP
Local refinement of the SARS-CoV-2 BA.2.86 RBD in complex with TRI2-2 minibinder
Summary for 9CWP
| Entry DOI | 10.2210/pdb9cwp/pdb |
| EMDB information | 45969 |
| Descriptor | BA.2.86 spike protein RBD, Pan-variant miniprotein TRI2-2, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total) |
| Functional Keywords | sarbecoviruses, spike glycoprotein, structural genomic, inhibitor, viral protein, minibinder, structural genomics, seattle structural genomics center for infectious disease, ssgcid |
| Biological source | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV, SARS-CoV-2) More |
| Total number of polymer chains | 2 |
| Total formula weight | 39967.02 |
| Authors | Lee, J.,Veesler, D.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2024-07-29, release date: 2026-01-21) |
| Primary citation | Lee, J.,Case, J.B.,Park, Y.J.,Ravichandran, R.,Asarnow, D.,Tortorici, M.A.,Brown, J.T.,Sanapala, S.,Carter, L.,Baker, D.,Diamond, M.S.,Veesler, D. The computationally designed TRI2-2 miniprotein inhibitor protects against multiple SARS-CoV-2 Omicron variants. Commun Biol, 2026 Cited by PubMed Abstract: The continued evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has compromised neutralizing antibody responses elicited by prior infection or vaccination and abolished the utility of most monoclonal antibody therapeutics. We previously described a computationally-designed, homotrimeric miniprotein inhibitor, designated TRI2-2, that protects mice against pre-Omicron SARS-CoV-2 variants. Here, we show that TRI2-2 exhibits broadly neutralizing activity of SARS-CoV-2 variants and protects mice against BQ.1.1, XBB.1.5 and BA.2.86 challenge when administered intranasally post-exposure. The resistance of TRI2-2 to viral escape by most variants and the ability to deliver it directly to the upper airways highlight the potential of the multivalent miniprotein inhibitor as an alternative therapeutic modality. PubMed: 41519898DOI: 10.1038/s42003-025-09499-2 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.2 Å) |
Structure validation
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