9CPC

Atomic model of porcine brain ventricles cilia doublet microtubule (48-nm periodicity)

これはPDB形式変換不可エントリーです。

9CPC の概要

• エントリーDOI: 10.2210/pdb9cpc/pdb
• EMDBエントリー: 45802
• 分子名称: Armadillo repeat-containing protein 4 isoform 1, Cilia and flagella associated protein 276, Enkurin, ... (43 entities in total)
• 機能のキーワード: axoneme, cilia, microtubule, dynein, brain ventricle, doublet microtubule (dmt), structural protein
• 由来する生物種: Sus scrofa (pig); 詳細
• タンパク質・核酸の鎖数: 377
• 化学式量合計: 18796804.01

構造登録者

Sun, C.,Zeng, J.,Zhang, R. (登録日: 2024-07-18, 公開日: 2024-10-23, 最終更新日: 2025-03-19)

主引用文献

Leung, M.R.,Sun, C.,Zeng, J.,Anderson, J.R.,Niu, Q.,Huang, W.,Noteborn, W.E.M.,Brown, A.,Zeev-Ben-Mordehai, T.,Zhang, R.
Structural diversity of axonemes across mammalian motile cilia.
Nature, 637:1170-1177, 2025

PubMed Abstract: Reproduction, development and homeostasis depend on motile cilia, whose rhythmic beating is powered by a microtubule-based molecular machine called the axoneme. Although an atomic model of the axoneme is available for the alga Chlamydomonas reinhardtii, structures of mammalian axonemes are incomplete. Furthermore, we do not fully understand how molecular structures of axonemes vary across motile-ciliated cell types in the body. Here we use cryoelectron microscopy, cryoelectron tomography and proteomics to resolve the 96-nm modular repeat of axonemal doublet microtubules (DMTs) from both sperm flagella and epithelial cilia of the oviduct, brain ventricles and respiratory tract. We find that sperm DMTs are the most specialized, with epithelial cilia having only minor differences across tissues. We build a model of the mammalian sperm DMT, defining the positions and interactions of 181 proteins including 34 newly identified proteins. We elucidate the composition of radial spoke 3 and uncover binding sites of kinases associated with regeneration of ATP and regulation of ciliary motility. We discover a sperm-specific, axoneme-tethered T-complex protein ring complex (TRiC) chaperone that may contribute to construction or maintenance of the long flagella of mammalian sperm. We resolve axonemal dyneins in their prestroke states, illuminating conformational changes that occur during ciliary movement. Our results illustrate how elements of chemical and mechanical regulation are embedded within the axoneme, providing valuable resources for understanding the aetiology of ciliopathy and infertility, and exemplifying the discovery power of modern structural biology.

PubMed: 39743588
DOI: 10.1038/s41586-024-08337-5

実験手法

ELECTRON MICROSCOPY (3.65 Å)