9COP

Yeast RAVE bound to V-ATPase V1 complex

これはPDB形式変換不可エントリーです。

9COP の概要

• エントリーDOI: 10.2210/pdb9cop/pdb
• EMDBエントリー: 45788 46463
• 分子名称: V-type proton ATPase catalytic subunit A, Suppressor of kinetochore protein 1, MAGNESIUM ION, ... (12 entities in total)
• 機能のキーワード: v-atpase, rave, assembly, hydrolase
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 747203.59

構造登録者

Wang, H.,Rubinstein, J.L. (登録日: 2024-07-17, 公開日: 2024-11-20, 最終更新日: 2025-06-04)

主引用文献

Wang, H.,Tarsio, M.,Kane, P.M.,Rubinstein, J.L.
Structure of yeast RAVE bound to a partial V 1 complex.
Proc.Natl.Acad.Sci.USA, 121:e2414511121-e2414511121, 2024

PubMed Abstract: Vacuolar-type ATPases (V-ATPases) are membrane-embedded proton pumps that acidify intracellular compartments in almost all eukaryotic cells. Homologous with ATP synthases, these multisubunit enzymes consist of a soluble catalytic V subcomplex and a membrane-embedded proton-translocating V subcomplex. The V and V subcomplexes can undergo reversible dissociation to regulate proton pumping, with reassociation of V and V requiring the protein complex known as RAVE (regulator of the ATPase of vacuoles and endosomes). In the yeast , RAVE consists of subunits Rav1p, Rav2p, and Skp1p. We used electron cryomicroscopy (cryo-EM) to determine a structure of yeast RAVE bound to V. In the structure, RAVE is an L-shaped complex with Rav2p pointing toward the membrane and Skp1p distant from both the membrane and V. Only Rav1p interacts with V, binding to a region of subunit A not found in the corresponding ATP synthase subunit. When bound to RAVE, V is in a rotational state suitable for binding the free V complex, but in the structure, it is partially disrupted, missing five of its 16 subunits. Other than these missing subunits and the conformation of the inhibitory subunit H, the V complex with RAVE appears poised for reassembly with V.

PubMed: 39625975
DOI: 10.1073/pnas.2414511121

実験手法

ELECTRON MICROSCOPY (2.7 Å)