9CM8

UDP-GlcNAc 2-epimerase MnaA of Paenibacillus alvei

Summary for 9CM8

• Entry DOI: 10.2210/pdb9cm8/pdb
• Descriptor: UDP-N-acetylglucosamine 2-epimerase (non-hydrolyzing), DI(HYDROXYETHYL)ETHER (3 entities in total)
• Functional Keywords: epimerase, udp-glcnac, udp-mannac, sugar binding protein, isomerase
• Biological source: Paenibacillus alvei
• Total number of polymer chains: 2
• Total formula weight: 89133.18

Authors

Legg, M.S.G.,Mateyko, N.,Evans, S.V. (deposition date: 2024-07-13, release date: 2024-10-02, Last modification date: 2024-10-23)

Primary citation

Stefanovic, C.,Legg, M.S.G.,Mateyko, N.,Ender, J.J.,Kuvek, T.,Oostenbrink, C.,Schaffer, C.,Evans, S.V.,Hager-Mair, F.F.
Insights into structure and activity of a UDP-GlcNAc 2-epimerase involved in secondary cell wall polymer biosynthesis in Paenibacillus alvei.
Front Mol Biosci, 11:1470989-1470989, 2024

PubMed Abstract: S-layer anchoring in is enabled by a non-covalent interaction between an S-layer homology domain trimer and a secondary cell wall polymer (SCWP), ensuring the structural integrity of the bacterial cell wall. Within the SCWP repeat, pyruvylated ManNAc serves as the ligand and the UDP-GlcNAc-2-epimerase MnaA supplies UDP-ManNAc to SCWP biosynthesis.

PubMed: 39391870
DOI: 10.3389/fmolb.2024.1470989

Experimental method

X-RAY DIFFRACTION (2.2 Å)