9CE8

20S Proteasome core particle in complex with Ixazomib

9CE8 の概要

• エントリーDOI: 10.2210/pdb9ce8/pdb
• 関連するPDBエントリー: 9CE5 9CE7
• EMDBエントリー: 45494 45495 45496
• 分子名称: Proteasome subunit alpha, Proteasome subunit beta, [(1~{R})-1-[2-[[2,5-bis(chloranyl)phenyl]carbonylamino]ethanoylamino]-3-methyl-butyl]boronic acid (3 entities in total)
• 機能のキーワード: proteasome, proteolysis, antimicrobial protein
• 由来する生物種: Mycobacterium tuberculosis; 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 724044.31

構造登録者

Zeytuni, N.,Uday, A.B.,Vahidi, S.,Turner, M. (登録日: 2024-06-26, 公開日: 2025-03-12, 最終更新日: 2025-04-16)

主引用文献

Turner, M.,Uday, A.B.,Velyvis, A.,Rennella, E.,Zeytuni, N.,Vahidi, S.
Structural basis for allosteric modulation of M. tuberculosis proteasome core particle.
Nat Commun, 16:3138-3138, 2025

PubMed Abstract: The Mycobacterium tuberculosis (Mtb) proteasome system selectively degrades damaged or misfolded proteins and is crucial for the pathogen's survival within the host. Targeting the 20S core particle (CP) offers a viable strategy for developing tuberculosis treatments. The activity of Mtb 20S CP, like that of its eukaryotic counterpart, is allosterically regulated, yet the specific conformations involved have not been captured in high-resolution structures to date. Here, we use single-particle electron cryomicroscopy and H/D exchange mass spectrometry to determine the Mtb 20S CP structure in an auto-inhibited state that is distinguished from the canonical resting state by the conformation of switch helices at the α/β interface. The rearrangement of these helices collapses the S1 pocket, effectively inhibiting substrate binding. Biochemical experiments show that the Mtb 20S CP activity can be altered through allosteric sites far from the active site. Our findings underscore the potential of targeting allostery to develop antituberculosis therapeutics.

PubMed: 40169579
DOI: 10.1038/s41467-025-58430-0

実験手法

ELECTRON MICROSCOPY (2.61 Å)