9C8K

Rabbit Hemorrhagic Disease Virus reinitiation stimulating TURBS RNA bound to rabbit ribosome

これはPDB形式変換不可エントリーです。

9C8K の概要

• エントリーDOI: 10.2210/pdb9c8k/pdb
• EMDBエントリー: 45307
• 分子名称: Small ribosomal subunit protein uS2, uS4, S10_plectin domain-containing protein, ... (37 entities in total)
• 機能のキーワード: viral rna, ribosome, translation regulation, reinitiation, rna
• 由来する生物種: Oryctolagus cuniculus (rabbit); 詳細
• タンパク質・核酸の鎖数: 35
• 化学式量合計: 1252991.50

構造登録者

Sherlock, M.E.,Kieft, J.S. (登録日: 2024-06-12, 公開日: 2025-02-19)

主引用文献

Sherlock, M.E.,Langeberg, C.J.,Segar, K.E.,Kieft, J.S.
A conserved class of viral RNA structures regulates translation reinitiation through dynamic ribosome interactions.
Cell Rep, 44:115236-115236, 2025

PubMed Abstract: Certain viral RNAs encode proteins downstream of their main open reading frame, expressed through "termination-reinitiation" events. In some cases, structures located upstream of the first stop codon within these viral RNAs bind the ribosome, inhibiting ribosome recycling and inducing reinitiation. We used bioinformatics methods to identify new examples of viral reinitiation-stimulating RNAs and experimentally verified their secondary structure and function. We determined the structure of a representative viral RNA-ribosome complex using cryoelectron microscopy (cryo-EM). 3D classification and variability analyses reveal that the viral RNA structure can sample a range of conformations while remaining tethered to the ribosome, enabling the ribosome to find a reinitiation start site within a limited range of mRNA sequence. Evaluating the conserved features and constraints of this entire RNA class within the context of the cryo-EM reconstruction provides insight into mechanisms enabling reinitiation, a translation regulation strategy employed by many other viral and eukaryotic systems.

PubMed: 39893634
DOI: 10.1016/j.celrep.2025.115236

実験手法

ELECTRON MICROSCOPY (3.1 Å)