9C5G

Structure of R. leguminosarum CapW bound to single-stranded DNA

9C5G の概要

• エントリーDOI: 10.2210/pdb9c5g/pdb
• 分子名称: CapW, DNA (5'-D(P*TP*TP*T)-3'), SULFATE ION, ... (4 entities in total)
• 機能のキーワード: capw, wyl, hth-wyl, transcription factor, brxr, dna binding protein-dna complex, dna binding protein/dna
• 由来する生物種: Rhizobium leguminosarum; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 74561.02

構造登録者

Blankenchip, C.L.,Corbett, K.D. (登録日: 2024-06-06, 公開日: 2024-10-30, 最終更新日: 2025-01-01)

主引用文献

Blankenchip, C.L.,Corbett, K.D.
Bacterial WYL domain transcriptional repressors sense single-stranded DNA to control gene expression.
Nucleic Acids Res., 52:13723-13732, 2024

PubMed Abstract: Bacteria encode a wide array of immune systems to protect themselves against ubiquitous bacteriophages and foreign DNA elements. While these systems' molecular mechanisms are becoming increasingly well known, their regulation remains poorly understood. Here, we show that an immune system-associated transcriptional repressor of the wHTH-WYL-WCX family, CapW, directly binds single-stranded DNA to sense DNA damage and activate expression of its associated immune system. We show that CapW mediates increased expression of a reporter gene in response to DNA damage in a host cell. CapW directly binds single-stranded DNA by-products of DNA repair through its WYL domain, causing a conformational change that releases the protein from double-stranded DNA. In an Escherichia coli CBASS system with an integrated capW gene, we find that CapW-mediated transcriptional activation is important for this system's ability to prevent induction of a λ prophage. Overall, our data reveal the molecular mechanisms of WYL-domain transcriptional repressors, and provide an example of how bacteria can balance the protective benefits of carrying anti-phage immune systems against the inherent risk of these systems' aberrant activation.

PubMed: 39588753
DOI: 10.1093/nar/gkae1101

実験手法

X-RAY DIFFRACTION (2.77 Å)