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9C4Q

Crystal structure of wild-type arabidopsis thaliana acetohydroxyacid synthase in complex with newly designed herbicide FMO

This is a non-PDB format compatible entry.
Summary for 9C4Q
Entry DOI10.2210/pdb9c4q/pdb
Related5k2o
DescriptorAcetolactate synthase, chloroplastic, FORMIC ACID, SULFATE ION, ... (12 entities in total)
Functional Keywordsherbicide, resistance, ahas, als, ligase, transferase
Biological sourceArabidopsis thaliana (thale cress)
Total number of polymer chains1
Total formula weight67044.47
Authors
Cheng, Y.,Guddat, L.W. (deposition date: 2024-06-05, release date: 2024-10-23)
Primary citationCheng, Y.,Wang, Y.,Lonhienne, T.,Wang, J.G.,Guddat, L.W.
Crystal Structures of Arabidopsis thaliana Acetohydroxyacid Synthase in Complex with the Herbicide Triasulfuron and Two Analogues with Herbicidal Activity in Field Trials.
J.Agric.Food Chem., 2024
Cited by
PubMed Abstract: Triasulfuron is a commercial herbicide of the sulfonylurea family. This compound targets acetohydroxyacid synthase (AHAS, E.C. 2.2.1.6), the first enzyme in the branched chain amino acid biosynthesis pathway. Here, we have determined crystal structures of AHAS (AHAS) in complex with triasulfuron and two newly designed herbicidal compounds, identified as FMO and CMO, showing that their binding modes are subtly different. Kinetic studies showed all three compounds exhibit varying values, 0.192 ± 0.013 μM for triasulfuron, 0.086 ± 0.013 μM for FMO, and 1.448 ± 0.058 μM for CMO, but all are strong time-dependent accumulative inhibitors of AHAS. Apart from triasulfuron being a powerful herbicide with application rates of 10-15 g/ha in wheat fields, CMO and FMO are also herbicidal at 7.5-30 g/ha for barnyard grass. This study emphasizes that accumulative inhibition is an important factor that contributes to herbicidal activity.
PubMed: 39373624
DOI: 10.1021/acs.jafc.4c04990
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.52 Å)
Structure validation

226707

數據於2024-10-30公開中

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