9C28

Structure of endogenous Glutamine synthetase from rat model of Alzheimer's disease

9C28 の概要

• エントリーDOI: 10.2210/pdb9c28/pdb
• 関連するPDBエントリー: 9C21
• EMDBエントリー: 45137
• 分子名称: Glutamine synthetase, MANGANESE (II) ION (2 entities in total)
• 機能のキーワード: glutamine synthetase, ligase
• 由来する生物種: Rattus norvegicus (Norway rat)
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 424336.53

構造登録者

Khalili Samani, E.,Keszei, A.F.A.,Mazhab-Jafari, M.T. (登録日: 2024-05-30, 公開日: 2024-12-11, 最終更新日: 2025-01-15)

主引用文献

Samani, E.K.,Hasan, S.M.N.,Waas, M.,Keszei, A.F.A.,Xu, X.,Heydari, M.,Hill, M.E.,McLaurin, J.,Kislinger, T.,Mazhab-Jafari, M.T.
Unveiling the structural proteome of an Alzheimer's disease rat brain model.
Structure, 33:51-, 2025

PubMed Abstract: Studying native protein structures at near-atomic resolution in a crowded environment presents challenges. Consequently, understanding the structural intricacies of proteins within pathologically affected tissues often relies on mass spectrometry and proteomic analysis. Here, we utilized cryoelectron microscopy (cryo-EM) and the Build and Retrieve (BaR) method to investigate protein complexes' structural characteristics such as post-translational modification, active site occupancy, and arrested conformational state in Alzheimer's disease (AD) using brain lysate from a rat model (TgF344-AD). Our findings reveal novel insights into the architecture of these complexes, corroborated through mass spectrometry analysis. Interestingly, it has been shown that the dysfunction of these protein complexes extends beyond AD, implicating them in cancer, as well as other neurodegenerative disorders such as Parkinson's disease, Huntington's disease, and schizophrenia. By elucidating these structural details, our work not only enhances our understanding of disease pathology but also suggests new avenues for future approaches in therapeutic intervention.

PubMed: 39615488
DOI: 10.1016/j.str.2024.11.004

実験手法

ELECTRON MICROSCOPY (3.12 Å)