9C0I

Structure of the DRT2 reverse transcriptase in complex with its non-coding RNA

9C0I の概要

• エントリーDOI: 10.2210/pdb9c0i/pdb
• EMDBエントリー: 45085
• 分子名称: Reverse transcriptase/maturase family protein, DNA primer, DRT2 ncRNA, ... (5 entities in total)
• 機能のキーワード: reverse transcriptase, rolling circle amplification, phage defense, transferase-dna-rna complex, transferase/dna/rna
• 由来する生物種: Klebsiella pneumoniae; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 142322.53

構造登録者

Wilkinson, M.E.,Zhang, F. (登録日: 2024-05-25, 公開日: 2024-09-04, 最終更新日: 2025-05-21)

主引用文献

Wilkinson, M.E.,Li, D.,Gao, A.,Macrae, R.K.,Zhang, F.
Phage-triggered reverse transcription assembles a toxic repetitive gene from a noncoding RNA.
Science, 386:eadq3977-eadq3977, 2024

PubMed Abstract: Reverse transcription has frequently been co-opted for cellular functions and in prokaryotes is associated with protection against viral infection, but the underlying mechanisms of defense are generally unknown. Here, we show that in the DRT2 defense system, the reverse transcriptase binds a neighboring pseudoknotted noncoding RNA. Upon bacteriophage infection, a template region of this RNA is reverse transcribed into an array of tandem repeats that reconstitute a promoter and open reading frame, allowing expression of a toxic repetitive protein and an abortive infection response. Biochemical reconstitution of this activity and cryo-electron microscopy provide a molecular basis for repeat synthesis. Gene synthesis from a noncoding RNA is a previously unknown mode of genetic regulation in prokaryotes.

PubMed: 39208082
DOI: 10.1126/science.adq3977

実験手法

ELECTRON MICROSCOPY (2.91 Å)