9BTI

Rhesus Fab 40591-a.01 in complex with T250.4 RnS SOSIP Env

9BTI の概要

• エントリーDOI: 10.2210/pdb9bti/pdb
• EMDBエントリー: 44891
• 分子名称: Envelope glycoprotein gp41, 2-acetamido-2-deoxy-beta-D-glucopyranose, Envelope glycoprotein gp120, ... (10 entities in total)
• 機能のキーワード: hiv-1, sosip, vaccine, viral protein-immune system complex, v2-apex, multi-donor, shiv, immune system, viral protein/immune system
• 由来する生物種: Human immunodeficiency virus 1; 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 282337.95

構造登録者

Gorman, J.,Kwong, P.D. (登録日: 2024-05-15, 公開日: 2025-06-11, 最終更新日: 2025-10-01)

主引用文献

Roark, R.S.,Habib, R.,Gorman, J.,Li, H.,Connell, A.J.,Bonsignori, M.,Guo, Y.,Hogarty, M.P.,Olia, A.S.,Sowers, K.J.,Zhang, B.,Bibollet-Ruche, F.,Bylund, T.,Callaghan, S.,Carey, J.W.,Cerutti, G.,Harris, D.R.,He, W.,Lewis, E.,Liu, T.,Mason, R.D.,Qiao, Y.,Park, Y.,Rando, J.M.,Singh, A.,Wolff, J.J.,Lei, Q.P.,Louder, M.K.,Andrabi, R.,Doria-Rose, N.A.,Saunders, K.O.,Seaman, M.S.,Haynes, B.F.,Kulp, D.W.,Mascola, J.R.,Roederer, M.,Pierson, T.C.,Sheng, Z.,Hahn, B.H.,Shaw, G.M.,Kwong, P.D.,Shapiro, L.
Structural and genetic basis of HIV-1 envelope V2 apex recognition by rhesus broadly neutralizing antibodies.
J.Exp.Med., 222:-, 2025

PubMed Abstract: Broadly neutralizing antibodies targeting the V2 apex of HIV-1 envelope are desired as vaccine design templates, but few have been described. Here, we report 11 lineages of V2 apex-neutralizing antibodies from simian-human immunodeficiency virus (SHIV)-infected rhesus macaques and determine cryo-EM structures for 9. A single V2 apex-neutralizing lineage accounted for cross-clade breadth in most macaques, and somatic hypermutation relative to breadth was generally low, exemplified by antibody V033-a.01 with <5% nucleotide mutation and 37% breadth (208-strain panel). Envelope complex structures revealed eight different antibody classes (one multi-donor) and the complete repertoire of all five possible recognition topologies, recapitulating canonical human modes of apex insertion and C-strand hydrogen bonding. Despite this diversity in recognition, all rhesus-V2 apex antibodies were derived from reading frame two of the DH3-15*01 gene. Collectively, these results define-in rhesus-the structural and genetic basis of HIV-1 V2 apex recognition and demonstrate unprecedented structural plasticity of a highly selected immunogenetic element.

PubMed: 40824240
DOI: 10.1084/jem.20250638

実験手法

ELECTRON MICROSCOPY (4.14 Å)