9BRA

Intact V-ATPase State 2 and synaptophysin complex in mouse brain isolated synaptic vesicles

これはPDB形式変換不可エントリーです。

9BRA の概要

• エントリーDOI: 10.2210/pdb9bra/pdb
• EMDBエントリー: 44839
• 分子名称: V-type proton ATPase subunit E 1, V-type proton ATPase 21 kDa proteolipid subunit c'', V-type proton ATPase subunit d 1, ... (17 entities in total)
• 機能のキーワード: v-atpase, synaptic vesicle, membrane protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 33
• 化学式量合計: 1081501.71

構造登録者

Wang, C.,Jiang, W.,Yang, K.,Wang, X.,Guo, Q.,Brunger, A.T. (登録日: 2024-05-11, 公開日: 2024-06-19, 最終更新日: 2024-08-07)

主引用文献

Wang, C.,Jiang, W.,Leitz, J.,Yang, K.,Esquivies, L.,Wang, X.,Shen, X.,Held, R.G.,Adams, D.J.,Basta, T.,Hampton, L.,Jian, R.,Jiang, L.,Stowell, M.H.B.,Baumeister, W.,Guo, Q.,Brunger, A.T.
Structure and topography of the synaptic V-ATPase-synaptophysin complex.
Nature, 631:899-904, 2024

PubMed Abstract: Synaptic vesicles are organelles with a precisely defined protein and lipid composition, yet the molecular mechanisms for the biogenesis of synaptic vesicles are mainly unknown. Here, we discovered a well-defined interface between the synaptic vesicle V-ATPase and synaptophysin by in situ cryo-electron tomography and single particle cryo-electron microscopy of functional synaptic vesicles isolated from mouse brains. The synaptic vesicle V-ATPase is an ATP-dependent proton pump that establishes the protein gradient across the synaptic vesicle, which in turn drives the uptake of neurotransmitters. Synaptophysin and its paralogs synaptoporin and synaptogyrin belong to a family of abundant synaptic vesicle proteins whose function is still unclear. We performed structural and functional studies of synaptophysin knockout mice, confirming the identity of synaptophysin as an interaction partner with the V-ATPase. Although there is little change in the conformation of the V-ATPase upon interaction with synaptophysin, the presence of synaptophysin in synaptic vesicles profoundly affects the copy number of V-ATPases. This effect on the topography of synaptic vesicles suggests that synaptophysin assists in their biogenesis. In support of this model, we observed that synaptophysin knockout mice exhibit severe seizure susceptibility, suggesting an imbalance of neurotransmitter release as a physiological consequence of the absence of synaptophysin.

PubMed: 38838737
DOI: 10.1038/s41586-024-07610-x

実験手法

ELECTRON MICROSCOPY (4.3 Å)