9BOR

IkappaBzeta ankyrin repeat domain:NF-kappaB p50 homodimer complex at 2.0 Angstrom resolution

Summary for 9BOR

• Entry DOI: 10.2210/pdb9bor/pdb
• Related: 4TRV
• Descriptor: NF-kappa-B inhibitor zeta, Nuclear factor NF-kappa-B p50 subunit, (2R,3S)-heptane-1,2,3-triol, ... (4 entities in total)
• Functional Keywords: transcription factor, immunoglobulin-like domain, ankyrin repeat domain, protein-protein complex, nf-kappab, ikappab, transcription
• Biological source: Homo sapiens (human); More
• Total number of polymer chains: 3
• Total formula weight: 65376.20

Authors

Rogers, W.E.,Zhu, N.,Huxford, T. (deposition date: 2024-05-05, release date: 2024-08-07)

Primary citation

Zhu, N.,Rogers, W.E.,Heidary, D.K.,Huxford, T.
Structural and biochemical analyses of the nuclear I kappa B zeta protein in complex with the NF-kappa B p50 homodimer.
Genes Dev., 38:528-535, 2024

PubMed Abstract: As part of the efforts to understand nuclear IκB function in NF-κB-dependent gene expression, we report an X-ray crystal structure of the IκBζ ankyrin repeat domain in complex with the dimerization domain of the NF-κB p50 homodimer. IκBζ possesses an N-terminal α helix that conveys domain folding stability. Affinity and specificity of the complex depend on a small portion of p50 at the nuclear localization signal. The model suggests that only one p50 subunit supports binding with IκBζ, and biochemical experiments confirm that IκBζ associates with DNA-bound NF-κB p50:RelA heterodimers. Comparisons of IκBζ:p50 and p50:κB DNA complex crystallographic models indicate that structural rearrangement is necessary for ternary complex formation of IκBζ and p50 with DNA.

PubMed: 38960718
DOI: 10.1101/gad.351892.124

Experimental method

X-RAY DIFFRACTION (1.997 Å)