9BLL

Cryo-EM of RBD(EG5.1)/1301B7 Fab Complex

9BLL の概要

• エントリーDOI: 10.2210/pdb9bll/pdb
• EMDBエントリー: 44666
• 分子名称: 1301B7 Heavy Chain, 1301B7 Light Chain, Spike glycoprotein, ... (5 entities in total)
• 機能のキーワード: fab, rbd, complex, protein binding, viral protein-immune system complex, viral protein/immune system
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 163865.83

構造登録者

Walter, M.R.,Green, T.J. (登録日: 2024-04-30, 公開日: 2025-03-12)

主引用文献

Piepenbrink, M.S.,Khalil, A.M.,Chang, A.,Mostafa, A.,Basu, M.,Sarkar, S.,Panjwani, S.,Ha, Y.H.,Ma, Y.,Ye, C.,Wang, Q.,Green, T.J.,Kizziah, J.L.,Erdmann, N.B.,Goepfert, P.A.,Liu, L.,Ho, D.D.,Martinez-Sobrido, L.,Walter, M.R.,Kobie, J.J.
Potent neutralization by a RBD antibody with broad specificity for SARS-CoV-2 JN.1 and other variants.
Npj Viruses, 2:55-55, 2024

PubMed Abstract: SARS-CoV-2 continues to be a public health burden, driven in-part by its continued antigenic diversification and resulting emergence of new variants. By increasing herd immunity, current vaccines have improved infection outcomes for many. However, prophylactic and treatment interventions that are not compromised by viral evolution of the Spike protein are still needed. Using a differential staining strategy with a rationally designed SARS-CoV-2 Receptor Binding Domain (RBD) - ACE2 fusion protein and a native Omicron RBD protein, we developed a recombinant human monoclonal antibody (hmAb) from a convalescent individual following SARS-CoV-2 Omicron infection. The resulting hmAb, 1301B7 potently neutralized a wide range of SARS-CoV-2 variants including the original Wuhan-1, the more recent Omicron JN.1 strain, and SARS-CoV. 1301B7 contacts the ACE2 binding site of RBD exclusively through its VH1-69 heavy chain. Broad specificity is achieved through 1301B7 binding to many conserved residues of Omicron variants including Y501 and H505. Consistent with its extensive binding epitope, 1301B7 is able to potently diminish viral burden in the upper and lower respiratory tract and protect mice from challenge with Omicron XBB1.5 and Omicron JN.1 viruses. These results suggest 1301B7 has broad potential to prevent or treat clinical SARS-CoV-2 infections and to guide development of RBD-based universal SARS-CoV-2 prophylactic vaccines and therapeutic approaches.

PubMed: 39553825
DOI: 10.1038/s44298-024-00063-z

実験手法

ELECTRON MICROSCOPY (4.1 Å)