9BDG

Influenza A virus Hemagglutinin H3/Darwin/6/2021 in complex with Fab ADI-85647

9BDG の概要

• エントリーDOI: 10.2210/pdb9bdg/pdb
• EMDBエントリー: 44452
• 分子名称: Hemagglutinin, Fab ADI-85647 Heavy chain, Ig-like domain-containing protein, ... (9 entities in total)
• 機能のキーワード: hemagglutinin, influenza a, antibody, fab, viral protein
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 331431.26

構造登録者

Ferreira Ramos, A.S.,Bajic, G. (登録日: 2024-04-11, 公開日: 2024-11-13, 最終更新日: 2025-05-28)

主引用文献

Maurer, D.P.,Vu, M.,Ferreira Ramos, A.S.,Dugan, H.L.,Khalife, P.,Geoghegan, J.C.,Walker, L.M.,Bajic, G.,Schmidt, A.G.
Conserved sites on the influenza H1 and H3 hemagglutinin recognized by human antibodies.
Sci Adv, 11:eadu9140-eadu9140, 2025

PubMed Abstract: Monoclonal antibodies (mAbs) targeting the influenza hemagglutinin (HA) can be used as prophylactics or templates for next-generation vaccines. Here, we isolated broad, subtype-neutralizing mAbs from human B cells recognizing the H1 or H3 HA "head" and a mAb engaging the conserved stem. The H1 mAbs bind the lateral patch epitope on HAs from 1933 to 2021 and a prepandemic swine H1N1 virus. We improved neutralization potency using directed evolution toward a contemporary H1 HA. Deep mutational scanning of four antigenically distinct H1N1 viruses identified potential viral escape pathways. For the H3 mAbs, we used cryo-electron microscopy to define their epitopes: One mAb binds the side of the HA head, accommodating the N133 glycan and a pocket underneath the receptor binding site; the other mAb recognizes an HA stem epitope that partially overlaps with previously characterized mAbs but with distinct antibody variable genes. Collectively, these mAbs identify conserved sites recognized by broadly-reactive mAbs that may be elicited by next-generation vaccines.

PubMed: 40267182
DOI: 10.1126/sciadv.adu9140

実験手法

ELECTRON MICROSCOPY (3.01 Å)