9BCT

Cryo-EM structure of Thermococcus kodakarensis FttA-dependent transcription pre-termination complex containing 44 nt RNA

9BCT の概要

• エントリーDOI: 10.2210/pdb9bct/pdb
• EMDBエントリー: 44438
• 分子名称: DNA-directed RNA polymerase subunit A', DNA-directed RNA polymerase subunit N, DNA-directed RNA polymerase subunit P, ... (19 entities in total)
• 機能のキーワード: rna polymerase, pre-termination complex, ftta, archaea, transcription
• 由来する生物種: Thermococcus kodakarensis; 詳細
• タンパク質・核酸の鎖数: 18
• 化学式量合計: 589219.59

構造登録者

You, L.,Ebright, R.H. (登録日: 2024-04-09, 公開日: 2024-07-31, 最終更新日: 2024-11-13)

主引用文献

You, L.,Wang, C.,Molodtsov, V.,Kuznedelov, K.,Miao, X.,Wenck, B.R.,Ulisse, P.,Sanders, T.J.,Marshall, C.J.,Firlar, E.,Kaelber, J.T.,Santangelo, T.J.,Ebright, R.H.
Structural basis of archaeal FttA-dependent transcription termination.
Nature, 635:229-236, 2024

PubMed Abstract: The ribonuclease FttA (also known as aCPSF and aCPSF1) mediates factor-dependent transcription termination in archaea. Here we report the structure of a Thermococcus kodakarensis transcription pre-termination complex comprising FttA, Spt4, Spt5 and a transcription elongation complex (TEC). The structure shows that FttA interacts with the TEC in a manner that enables RNA to proceed directly from the TEC RNA-exit channel to the FttA catalytic centre and that enables endonucleolytic cleavage of RNA by FttA, followed by 5'→3' exonucleolytic cleavage of RNA by FttA and concomitant 5'→3' translocation of FttA on RNA, to apply mechanical force to the TEC and trigger termination. The structure further reveals that Spt5 bridges FttA and the TEC, explaining how Spt5 stimulates FttA-dependent termination. The results reveal functional analogy between bacterial and archaeal factor-dependent termination, functional homology between archaeal and eukaryotic factor-dependent termination, and fundamental mechanistic similarities in factor-dependent termination in bacteria, archaea, and eukaryotes.

PubMed: 39322680
DOI: 10.1038/s41586-024-07979-9

実験手法

ELECTRON MICROSCOPY (2.5 Å)