9B03

INF2 in the Middle of F-Actin (Up state)

9B03 の概要

• エントリーDOI: 10.2210/pdb9b03/pdb
• EMDBエントリー: 44026
• 分子名称: Actin, alpha skeletal muscle, Inverted formin-2, ADENOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: actin, formin, filament, severing, cytosolic protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 350488.80

構造登録者

Palmer, N.J.,Barrie, K.R.,Dominguez, R. (登録日: 2024-03-11, 公開日: 2024-05-29, 最終更新日: 2024-08-21)

主引用文献

Palmer, N.J.,Barrie, K.R.,Dominguez, R.
Mechanisms of actin filament severing and elongation by formins.
Nature, 632:437-442, 2024

PubMed Abstract: Humans express 15 formins that play crucial roles in actin-based processes, including cytokinesis, cell motility and mechanotransduction. However, the lack of structures bound to the actin filament (F-actin) has been a major impediment to understanding formin function. Whereas formins are known for their ability to nucleate and elongate F-actin, some formins can additionally depolymerize, sever or bundle F-actin. Two mammalian formins, inverted formin 2 (INF2) and diaphanous 1 (DIA1, encoded by DIAPH1), exemplify this diversity. INF2 shows potent severing activity but elongates weakly whereas DIA1 has potent elongation activity but does not sever. Using cryo-electron microscopy (cryo-EM) we show five structural states of INF2 and two of DIA1 bound to the middle and barbed end of F-actin. INF2 and DIA1 bind differently to these sites, consistent with their distinct activities. The formin-homology 2 and Wiskott-Aldrich syndrome protein-homology 2 (FH2 and WH2, respectively) domains of INF2 are positioned to sever F-actin, whereas DIA1 appears unsuited for severing. These structures also show how profilin-actin is delivered to the fast-growing barbed end, and how this is followed by a transition of the incoming monomer into the F-actin conformation and the release of profilin. Combined, the seven structures presented here provide step-by-step visualization of the mechanisms of F-actin severing and elongation by formins.

PubMed: 38843827
DOI: 10.1038/s41586-024-07637-0

実験手法

ELECTRON MICROSCOPY (2.95 Å)