9AZ7

Chloride Sites in Photoactive Yellow Protein

9AZ7 の概要

• エントリーDOI: 10.2210/pdb9az7/pdb
• 分子名称: Photoactive yellow protein, 4'-HYDROXYCINNAMIC ACID, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: photoreceptor, light sensor, chromophore, photoreceptor protein, receptor, sensory transduction, signaling protein, pas, lov
• 由来する生物種: Halorhodospira halophila
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14159.09

構造登録者

Dyda, F.,Schotte, F.,Anfinrud, P.,Cho, H.S. (登録日: 2024-03-10, 公開日: 2024-03-20, 最終更新日: 2024-04-24)

主引用文献

Schotte, F.,Cho, H.S.,Dyda, F.,Anfinrud, P.
Watching a signaling protein function: What has been learned over four decades of time-resolved studies of photoactive yellow protein.
Struct Dyn., 11:021303-021303, 2024

PubMed Abstract: Photoactive yellow protein (PYP) is a signaling protein whose internal p-coumaric acid chromophore undergoes reversible, light-induced -to- isomerization, which triggers a sequence of structural changes that ultimately lead to a signaling state. Since its discovery nearly 40 years ago, PYP has attracted much interest and has become one of the most extensively studied proteins found in nature. The method of time-resolved crystallography, pioneered by Keith Moffat, has successfully characterized intermediates in the PYP photocycle at near atomic resolution over 12 decades of time down to the sub-picosecond time scale, allowing one to stitch together a movie and literally watch a protein as it functions. But how close to reality is this movie? To address this question, results from numerous complementary time-resolved techniques including x-ray crystallography, x-ray scattering, and spectroscopy are discussed. Emerging from spectroscopic studies is a general consensus that three time constants are required to model the excited state relaxation, with a highly strained ground-state intermediate formed in less than 2.4 ps. Persistent strain drives the sequence of structural transitions that ultimately produce the signaling state. Crystal packing forces produce a restoring force that slows somewhat the rates of interconversion between the intermediates. Moreover, the solvent composition surrounding PYP can influence the number and structures of intermediates as well as the rates at which they interconvert. When chloride is present, the PYP photocycle in a crystal closely tracks that in solution, which suggests the epic movie of the PYP photocycle is indeed based in reality.

PubMed: 38595979
DOI: 10.1063/4.0000241

実験手法

X-RAY DIFFRACTION (2 Å)