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9AYR

Structure of a Ric1-Rgp1-Rab6 activation intermediate

9AYR の概要
エントリーDOI10.2210/pdb9ayr/pdb
EMDBエントリー43997
分子名称Guanine nucleotide exchange factor subunit RIC1, Guanine nucleotide exchange factor subunit RGP1, GTP-binding protein YPT6 (3 entities in total)
機能のキーワードgef, gtpase, rab, protein transport
由来する生物種Saccharomyces cerevisiae (brewer's yeast)
詳細
タンパク質・核酸の鎖数3
化学式量合計219344.88
構造登録者
Feathers, J.R.,Fromme, J.C. (登録日: 2024-03-08, 公開日: 2024-12-11, 最終更新日: 2025-05-28)
主引用文献Feathers, J.R.,Vignogna, R.C.,Fromme, J.C.
Structural basis for Rab6 activation by the Ric1-Rgp1 complex.
Nat Commun, 15:10561-10561, 2024
Cited by
PubMed Abstract: Rab GTPases act as molecular switches to regulate organelle homeostasis and membrane trafficking. Rab6 plays a central role in regulating cargo flux through the Golgi and is activated via nucleotide exchange by the Ric1-Rgp1 protein complex. Ric1-Rgp1 is conserved throughout eukaryotes but the structural and mechanistic basis for its function has not been established. Here we report the cryoEM structure of a Ric1-Rgp1-Rab6 complex representing a key intermediate of the nucleotide exchange reaction. Ric1-Rgp1 interacts with the nucleotide-binding domain of Rab6 using an uncharacterized helical domain, which we establish as a RabGEF domain by identifying residues required for Rab6 activation. Unexpectedly, the complex uses an arrestin fold to interact with the Rab6 hypervariable domain, indicating that interactions with the unstructured C-terminal regions of Rab GTPases may be a common binding mechanism used by their activators. Collectively, our findings provide a detailed mechanistic understanding of regulated Rab6 activation at the Golgi.
PubMed: 39632878
DOI: 10.1038/s41467-024-54869-9
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (3.3 Å)
構造検証レポート
Validation report summary of 9ayr
検証レポート(詳細版)ダウンロードをダウンロード

238895

件を2025-07-16に公開中

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