9AYR
Structure of a Ric1-Rgp1-Rab6 activation intermediate
9AYR の概要
エントリーDOI | 10.2210/pdb9ayr/pdb |
EMDBエントリー | 43997 |
分子名称 | Guanine nucleotide exchange factor subunit RIC1, Guanine nucleotide exchange factor subunit RGP1, GTP-binding protein YPT6 (3 entities in total) |
機能のキーワード | gef, gtpase, rab, protein transport |
由来する生物種 | Saccharomyces cerevisiae (brewer's yeast) 詳細 |
タンパク質・核酸の鎖数 | 3 |
化学式量合計 | 219344.88 |
構造登録者 | |
主引用文献 | Feathers, J.R.,Vignogna, R.C.,Fromme, J.C. Structural basis for Rab6 activation by the Ric1-Rgp1 complex. Nat Commun, 15:10561-10561, 2024 Cited by PubMed Abstract: Rab GTPases act as molecular switches to regulate organelle homeostasis and membrane trafficking. Rab6 plays a central role in regulating cargo flux through the Golgi and is activated via nucleotide exchange by the Ric1-Rgp1 protein complex. Ric1-Rgp1 is conserved throughout eukaryotes but the structural and mechanistic basis for its function has not been established. Here we report the cryoEM structure of a Ric1-Rgp1-Rab6 complex representing a key intermediate of the nucleotide exchange reaction. Ric1-Rgp1 interacts with the nucleotide-binding domain of Rab6 using an uncharacterized helical domain, which we establish as a RabGEF domain by identifying residues required for Rab6 activation. Unexpectedly, the complex uses an arrestin fold to interact with the Rab6 hypervariable domain, indicating that interactions with the unstructured C-terminal regions of Rab GTPases may be a common binding mechanism used by their activators. Collectively, our findings provide a detailed mechanistic understanding of regulated Rab6 activation at the Golgi. PubMed: 39632878DOI: 10.1038/s41467-024-54869-9 主引用文献が同じPDBエントリー |
実験手法 | ELECTRON MICROSCOPY (3.3 Å) |
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